中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 蛋黄磷脂酶解进程的测定 食品科学 2009,30 (7) 114-117
Position: Home > Articles > Determination of Enzymatic Hydrolysis Process of Egg Yolk Phospholipid FOOD SCIENCE 2009,30 (7) 114-117

蛋黄磷脂酶解进程的测定

作  者：
王迎新;迟玉杰
单  位：
东北农业大学食品学院
关键词：
蛋黄;磷脂酶A2;Dole法;游离脂肪酸;高效液相色谱;磷脂酰胆碱
摘  要：
食品加工中鸡蛋蛋黄在经高温处理后乳化性会显著下降,采用酶法改性可提高其乳化性和热稳定性。为监控磷脂酶水解蛋黄的反应进程,本研究采用改进后的Dole法对水解产物游离脂肪酸(FFA)进行测定,并比较了改进的Dole法与高效液相色谱法(HPLC)在监控水解反应进程上的相关性。结果表明,用Dole法测定反应生成的FFA量与用HPLC法测定磷脂酰胆碱(PC)的减少量呈良好的线性关系,相关系数为0.999,验证了用Dole法来监控反应进程的可行性,为实际应用建立了一种快速、简便、准确的方法。
译  名：
Determination of Enzymatic Hydrolysis Process of Egg Yolk Phospholipid
作  者：
WANG Ying-xin,CHI Yu-jie (College of Food, Northeast Agricultural University, Harbin 150030, China)
关键词：
egg yolk;phospholipase A2;Dole method;free fatty acids (FFA);HPLC;phosphatidylcholine
摘  要：
The emulsifying property of egg yolk decreases sharply in high temperature processing of food. Enzymatic method can improve the emulsifying property and thermal stability of egg yolk. In order to monitor the hydrolysis reaction, the hydrolysis products, free fatty acids (FFA) were determined by improved Dole method. Moreover, the correlation between the improved Dole method and high-performance liquid chromatography (HPLC) in monitoring the hydrolysis process was also investigated. The results showed that there is a good linear relationship between the generation of FFA determined by Dole method and the reduction of phosphatidylcholine determined by HPLC with a correlation coefficient of 0.999. And the feasibility of Dole method was verified for monitoring the reaction process. In conclusion, the Dole method is a fast, simple and accurate way.

相似文章

导出引用
计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46