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Position: Home > Articles > Effects of Cold on the Non-specific Immune Response in Hoplobatrachus rugulosus Journal of Anhui Agricultural Sciences 2010,38 (23) 12486-12487+12490

冷暴露对虎纹蛙非特异性免疫应答的影响

作  者:
王存盼;颉志刚;李小玲
单  位:
浙江师范大学生态研究所
关键词:
冷暴露;虎纹蛙;非特异性免疫
摘  要:
[目的]探讨冷暴露对虎纹蛙非特异性免疫应答的影响。[方法]将体重为(47.76±1.10)g的虎纹蛙分别在22℃和10℃条件下暴露10d,比较脾巨噬细胞呼吸爆发强度、血清补体活力、胃溶菌酶活力3项免疫指标在注射灭活大肠杆菌(Escherichia coli)12h后的应答情况。[结果]注射大肠杆菌前,10℃处理组血清补体活力显著低于22℃对照组(P<0.05),而2组间脾巨噬细胞呼吸爆发强度及胃溶菌酶活力无显著性差异;注射大肠杆菌后,10℃处理组的3项免疫指标对大肠杆菌刺激的应答能力变化均不显著,而22℃对照组则变化显著(P<0.05)。[结论]冷暴露会导致虎纹蛙非特异性免疫应答降低。
译  名:
Effects of Cold on the Non-specific Immune Response in Hoplobatrachus rugulosus
作  者:
WANG Cun-pan et al (Institute of ecology,Zhejiang normal university,Jinhua,Zhejiang 321004)
关键词:
Cold exposure; Hoplobatrachus rugulosus ; Non-specific immune response
摘  要:
[Objective]To evaluate the effect of cold on the non-specific immune response in Hoplobatrachus rugulosus. [Methord] The response of non-specific immune indexes including respiratory burst of spleen cells,gastric lysozyme activity and serum complement activity to the stimulation of heat-killed Escherichia coli were determined after being exposed at 22 ℃ and 10 ℃ for 10 d. [Result] The serum hemolysis activity of the frogs kept at 10 ℃ significantly decreased(P<0.05) while respiratory burst of spleen cells and gastric lysozyme activity didn't vary obviously,compared with those at 22 ℃. The three non-specific immune responses to bacteria stimulation changed significantly(P<0.05) after treated with E.coli at 22 ℃. [Conclusion] The non-specific immune response decreased after exposure to cold in Hoplobatrachus rugulosus.

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