中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 应用ISSR技术分析杧果畸形病病原菌的遗传多样性 广东农业科学 2014,41 (24) 79-82+87
Position: Home > Articles > Study on genetic diversity of Fusarium mangiferae from mango malformation disease by ISSR analysis Guangdong Agricultural Sciences 2014,41 (24) 79-82+87

应用ISSR技术分析杧果畸形病病原菌的遗传多样性

作  者：
柳凤;欧雄常;韦继光;詹儒林;常金梅
单  位：
广西大学农学院;中国热带农业科学院南亚热带作物研究所/农业部热带果树生物学重点实验室
关键词：
杧果畸形病;ISSR;遗传多样性
摘  要：
为探索我国杧果畸形病病原菌的遗传多样性,运用ISSR分子标记技术分析了采自四川攀枝花市和云南华坪县的38个杧果畸形病病原菌的遗传多样性。供试的38个菌株经14条特异性引物共扩增出72条带,其中52条带具有多态性,多态性比率为72.22%。38个菌株的相似系数值为0.5972~0.9862,变幅为0.3889,平均为0.7885。其中菌株MG16与MG17的相似系数最大、为0.9862;菌株MG07和MG33的遗传相似系数最小、为0.5972。利用UPGMA法进行聚类分析,38株菌株在相似系数为0.76水平上分为5类,菌株MG33、MG07和MG36分别形成单一分支,MG25和MG35聚合在一起;其余菌株分布在第Ⅴ类中。我国杧果畸形病病原菌Fusarium mangiferae存在丰富的种资资源,且F.mangiferae与采集地点、杧果品种之间无明显相关性。
译  名：
Study on genetic diversity of Fusarium mangiferae from mango malformation disease by ISSR analysis
作  者：
LIU Feng;OU Xiong-chang;WEI Ji-guang;ZHAN Ru-lin;CHANG Jin-mei;South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Tropical Fruit Biology,Ministry of Agriculture;Agricultural College, Guangxi University;
关键词：
mango malformation disease;;interal simple sequence repeat;;genetic diversity
摘  要：
In order to explicit the genetic diversity of 38 strains Fusarium mangiferae from mango malformation disease from Panzhihua, Sichuan, and Huaping, Yunnan in China, the ISSR technique was used. 72 bands were amplified by 14 ISSR primers, with an average of 5.14 bands per primer. The size of the amplified products ranged from 300 bp to2 000 bp. The genetic similarities among isolates ranged from 0.5972 to 0.9862. Isolates MG16 and MG17 were the most similar(0.9862 genetic similarity), whereas isolates MG07 and MG33 were the least similar(0.5972) and were grouped in separate clusters. At a genetic similarity of 0.76, the unweighted pair-group method with arithmetic mean analysis separated the isolates into five distinct clusters, comprising 33, two, one, one and one isolate, respectively. The ISSR data supported the conclusion that the isolates of F. mangiferae were not necessarily related by geographic origin, type of tissue or the mango cultivar from which they were isolated. This is the first report on the diversity of F. mangiferae species associated with mango malformation in China.

相似文章

导出引用
计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46