译 名：
Separation of Major Royal Jelly Protein 1 Using Different Hydrophobic Chromatographic Columns
作 者：
WANG Xueyu;QIAO Jiangtao;LIU Yinkang;ZHANG Hongcheng;DONG Jie;Institute of Apicultural Research, Chinese Academy of Agricultural Sciences;College of Food Science, Harbin University of Commerce;National Research Center of Bee Product Processing, Ministry of Agriculture;
关键词：
major royal jelly protein 1;;hydrophobic chromatography column;;native gel electrophoresis;;protein separation
摘 要：
Objective: To make a comparison of different hydrophobic chromatography columns for separating major royal jelly protein 1(MRJP 1), and choose a suitable one to separate MRJP 1 oligomer and MRJP 1 monomer. Methods: Totally 12 kinds of hydrophobic chromatography columns were applied to separate crude royal jelly protein, then the MRJP 1 oligomer and MRJP 1 monomer fractions were verified by native gel electrophoresis. Results: MRJP 1 was completely isolated by hydrophobic interaction chromatography on Hitrap Butyl HP, and an MRJP 1 oligomer fraction and an MRJP 1 monomer fraction were obtained. Conclusion: Hydrophobic chromatographic column provides an effective approach to separate MRJP 1 from royal jelly.
