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当前位置: 首页 > 文章 > 家蚕体形突变新缢蚕(co-n)的遗传分析与基因定位研究 蚕业科学 2009,35 (1) 13-17
Position: Home > Articles > Genetic Analysis and Gene Localization of a Body Shape Mutant Named New Constricted in the Silkworm,Bombyx mori Science of Sericulture 2009,35 (1) 13-17

家蚕体形突变新缢蚕(co-n)的遗传分析与基因定位研究

作  者：
代方银;童晓玲;胡海;谭端;黄飞飞;鲁成;向仲怀
单  位：
西南大学生物技术学院
关键词：
家蚕;突变;体形;新缢蚕;遗传分析;基因定位
摘  要：
从西南大学家蚕基因库中发现体形自然突变"新缢蚕"。幼虫表型特征为:环节中央部位呈缢缩状,色素聚集,腹部节间膜呈隆起状,5龄中后期最明显,与缢蚕(constricted,co)相似。至成虫期观察,腹背通常少有鳞毛。经遗传分析,新缢蚕为隐性基因支配,将其命名为new constricted,基因符号:co-n。连锁分析结果表明,co-n与黄血(Y)基因连锁,故判定该基因属于第2连锁群。这是家蚕第2连锁群30多个突变中发现的第一个体形突变。经过新缢蚕(co-n)与灰色卵(Gr)、黄血(Y)基因间的三点测验定位实验,结合伴野丰2005年最新公布的家蚕连锁遗传图的第2连锁群基因座数据,将co-n基因座确定为:2-5.7。
译  名：
Genetic Analysis and Gene Localization of a Body Shape Mutant Named New Constricted in the Silkworm,Bombyx mori
作  者：
DAI Fang-Yin1 TONG Xiao-Ling1,2 HU Hai1 TAN Duan1HUANG Fei-Fei1 LU Cheng1 XIANG Zhong-Huai1(1College of Biotechnology,Institute of Sericulture and System Biology,Southwest University,Chongqing 400716,China; 2Institute of Agriculture and Life Science,Chongqing University,Chongqing 400030,China)
关键词：
Bombyx mori;Mutant;Body shape;New constricted;Genetic analysis;Gene localization
摘  要：
"New constricted" is a natural mutant of body shape discovered in the silkworm germplasm bank at Southwest University.Its larvae are characterized with constriction and pigment deposition in the center of body segments,and bulge at the abdominal inter-segmental membrane.These characters are especially obvious at later days of the fifth-instar,which make the larvae resemble those with the constriced(co) gene.Generally,the adults' dorsal and abdominal surfaces have very few scales.Genetic analysis showed that the new constricted appearance is controlled by a recessive mutant gene.It was named new constricted with the gene symbol co-n.Linkage analysis indicated that co-n is linked with yellow blood(Y) gene and belongs to the 2nd linkage group.It is the first body shape mutant among more than thirty mutants which have been discovered on the 2nd linkage group.The result of three-point test among new constricted(co-n),grey color egg(Gr) and yellow blood(Y),and data of the latest linkage map published by Banno Yutaka in 2005 showed that the gene locus of co-n is at 2-5.7.

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