当前位置: 首页 > 文章 > 诱导物对出芽短梗霉木聚糖酶活力和阿魏酰低聚糖合成的调控影响 食品科学 2012,33 (1) 209-214
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诱导物对出芽短梗霉木聚糖酶活力和阿魏酰低聚糖合成的调控影响

作  者:
张雨青;余晓红;顾振新;屠康
单  位:
南京农业大学食品科技学院;盐城工学院化学与生物工程学院
关键词:
麦麸;阿魏酰低聚糖;诱导物;合成调控;出芽短梗霉
摘  要:
以麦麸为原料诱变选育的不产黑色素出芽短梗霉为发酵菌株,利用其发酵过程中合成的木聚糖酶水解麦麸纤维,制备阿魏酰低聚糖(FOs)。研究碳源、氮源、金属离子和表面活性剂等诱导物对出芽短梗霉木聚糖酶活力和FOs合成的影响,以探明各物质对出芽短梗霉木聚糖酶活力和FOs合成的影响;利用正交试验设计方法研究各物质添加量对出芽短梗霉产酶和FOs的影响,确定芽短梗霉发酵制备FOs的最佳培养基。结果表明:50g/L麦麸处理液中添加15g/L葡萄糖、1g/L蛋白胨和1g/L玉米浆,FOs产量和木聚糖酶活力最高分别达到627nmol/L和52.66IU/mL。
译  名:
Effect of Inducers on Xylanase Activity and Biosynthesis of Ferulic Oligosaccharides in Aureobasidium pullulans
作  者:
ZHANG Yu-qing1,YU Xiao-hong1,2,,GU Zhen-xin2,TU Kang2(1.College of Chemistry and Biological Engineering,Yancheng Institute of Technology,Yancheng 224003,China; 2.College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)
关键词:
wheat bran;ferulic oligosaccharides;inducer;biosynthesis and control;Aureobasidium pullulans
摘  要:
Feruloyl oligosaccharides(FOs) could be produced as a result of the hydrolysis of wheat bran fiber by xylanase synthesized during fermentation by a mutant strain of Aureobasidium pullulans without the secretion of melanin bred using wheat bran.The effects of inducers such as carbon source,nitrogen source,metal ion and surfactant on the activity of xylanase and the formation of FOs were investigated.The medium composition for FOs production by the strain was optimized using the orthogonal array design method.The highest Fos production and xylanase activity,627 nmol/L and 52.66 IU/mL,respectively,were achieved by using a medium comprised of 50 g/L wheat bran,15 g/L glucose,1 g/L peptone and 1 g/L corn syrup.

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