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当前位置: 首页 > 文章 > 白酒中一种三肽Arg-Asn-His的鉴定及其细胞内抗氧化活性 食品科学 2018 (23) 126-133
Position: Home > Articles > Identification of a Tripeptide Arg-Asn-His from Chinese Baijiu and Its Antioxidant Activity FOOD SCIENCE 2018 (23) 126-133

白酒中一种三肽Arg-Asn-His的鉴定及其细胞内抗氧化活性

作  者：
霍嘉颖;孙宝国;郑福平;孙金沅;孙啸涛;李贺贺;罗雪莲;黄明泉
单  位：
北京工商大学食品学院食品营养与人类健康北京高精尖创新中心中国轻工业酒类品质与安全重点实验室;中国疾病预防控制中心传染病预防控制所
关键词：
白酒;多肽;高效液相色谱-四极杆-飞行时间质谱;抗氧化活性;人肝癌HepG2细胞
摘  要：
利用直接浓缩并结合液-液萃取从白酒中分离纯化出一种三肽,并通过高效液相色谱-四极杆-飞行时间质谱联用仪进行鉴定,确定其序列为Arg-Asn-His(RNH),质量浓度为(18.78±0.34)μg/L。采用合成的RNH标准品研究其对2,2’-偶氮双(2-甲基丙脒)二盐酸盐(2,2’-azobis(2-methyl propionamidine) dihydrochloride,AAPH)诱导的人体肝癌HepG2细胞氧化损伤的保护作用。结果表明,RNH对细胞内活性氧具有清除作用,其作用是通过增加细胞内抗氧化酶(过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶)的活力及细胞内还原性化合物谷胱甘肽浓度来完成的,同时其可以防止丙二醛含量的增加,降低细胞中AAPH诱导的脂质过氧化水平,从而提高细胞的抗氧化能力,且其抗氧化能力呈浓度依赖性增加。本研究结果能帮助人们更好地认识白酒与健康之间的关系。
译  名：
Identification of a Tripeptide Arg-Asn-His from Chinese Baijiu and Its Antioxidant Activity
作  者：
HUO Jiaying;SUN Baoguo;ZHENG Fuping;SUN Jinyuan;SUN Xiaotao;LI Hehe;LUO Xuelian;HUANG Mingquan;Beijing Advanced Innovation Centre for Food Nutrition and Human Health, State Key Laboratory of Light Industrial Liquor Quality and Safety, School of Food and Chemical Engineering, Beijing Technology and Business University;National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention;
单  位：
HUO Jiaying%SUN Baoguo%ZHENG Fuping%SUN Jinyuan%SUN Xiaotao%LI Hehe%LUO Xuelian%HUANG Mingquan%Beijing Advanced Innovation Centre for Food Nutrition and Human Health, State Key Laboratory of Light Industrial Liquor Quality and Safety, School of Food and Chemical Engineering, Beijing Technology and Business University%National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention
关键词：
Chinese Baijiu;;peptide;;high performance liquid chromatograph-quadrupole-time-of-flight mass spectrometry;;antioxidant activity;;HepG2 cell
摘  要：
In this paper, a novel tripeptide was isolated at a concentration of(18.78 ± 0.34) μg/L from Chinese Baijiu by direct condensation, and its structure was identified as Arg-Asn-His(RNH) by high performance liquid chromatographquadrupole-time-of-flight mass spectrometry. The synthetic RNH was used to explore the protective effect of RNH on oxidative damage induced by 2,2'-azobis(2-methyl propionamidine) dihydrochloride(AAPH) in human hepatoma cells(HepG2). The results showed that RNH had a potent scavenging effect against intracellular reactive oxygen species by increasing the activities of antioxidant enzymes(catalase, superoxide dismutase and glutathione peroxidase) and the level of reduced glutathione in the cells. In addition, it prevented the increase of malondialdehyde level and decreased AAPH-induced lipid peroxidation level, thereby enhancing cellular antioxidant defense capacity. The antioxidant activity of the peptide was dose dependent. The results of this study can provide better understanding of the relationship between Chinese Baijiu and health.

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