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当前位置: 首页 > 文章 > 香蕉枯萎病菌fga1基因克隆及其多样性研究 分子植物育种 2011,09 (6) 62-68
Position: Home > Articles > Cloning and Diversity of fga1 from Fusarium oxysporum f. sp. cubense Molecular Plant Breeding 2011,09 (6) 62-68

香蕉枯萎病菌fga1基因克隆及其多样性研究

作  者：
李春雨;陈石;孙清明;邝瑞彬;左存武;郑加协;周红玲;易干军
单  位：
福建省农业科学院闽台园艺研究中心;广东省农业科学院果树研究所
关键词：
香蕉枯萎病;生理小种;fga1基因;可变性剪切
摘  要：
尖孢镰刀菌古巴专化型(Fusarium oxysporum f.sp.cubense)是引起香蕉枯萎病的病原菌,其中1号生理小种(Foc1)侵染粉蕉品种,而4号生理小种(Foc4)则危害粉蕉和香牙蕉品种。为探讨Foc1和Foc4致病性差异的遗传基础,本文根据已经公布的番茄枯萎病菌(Fusarium oxysporum f.sp.radicis-lycopersici)基因组序列,利用同源克隆的方法,分离到一个重要的致病基因——fga1,比较了不同区域Foc1和Foc4fga1基因cDNA和gDNA序列的差异。结果表明Foc1中fga1基因保守性很强,含有3个内含子和4个外显子,蛋白产物含353个氨基酸;所研究材料中Foc4fga1基因80%都有2个转录本,小转录本与Foc1fga1一致,而大转录本第三个内含子由于可变性剪切使得其成为外显子,预测编码一个372个氨基酸的多肽;来自海南三亚的Foc1和Foc4的fga1基因gDNA和cDNA长度分别为1286bp和1092bp,相似性均为100%。
译  名：
Cloning and Diversity of fga1 from Fusarium oxysporum f. sp. cubense
作  者：
Li Chunyun 1 Chen Shi 1,2 Sun Qingming 1 Kuang Ruibin 1 Zuo Cunwu 1 Zheng Jiaxie 2 Zhou Hongling 2 Yi Ganjun 1 1 Institution of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, 510640; 2 Fujian-Taiwan Horticulture Research Center, Fujian Academy of Agricultural Sciences, Zhangzhou, 363005
关键词：
Fusarium wilt of banana, Race, fga1 gene, Alternative splicing
摘  要：
Fusarium oxysporum f. sp. cubense is the causal agent of Fusarium wilt of banana, while Race 1 (Foc1) infects the Fenjiao cultivars, and beside Fenjiao, Race 4 (Foc4) also infects Cavendish bananas. Basing on the published genomic sequences of Fusarium oxysporum f. sp. radicis-lycopersici, an important pathogencity gene-fga1 was isolated using the method of homology-based cloning. Sequences of Foc1 and Foc4 from different regions were aligned. The results were shown that the full-length of gDNA of Foc1 and Foc4 from Hainan is 1 286 bp, and their sequence homology is 100%. The sequence of Foc1 fga1 was conserved, including 4 extrons and 3 introns, which encoded a protein with 353 amino acides. However, 80% of Foc4 fga1 had two transcripts, and the smaller one was same to Foc1 fga1, while the larger one encoded a polypeptide with 372 amino acids due to an alternative splicing making the third intron become an extron.

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