中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 小麦品种面筋强度、黄色素含量和PPO活性相关基因的分子检测 麦类作物学报 2008,28 (2) 220-226
Position: Home > Articles > Molecular Characterization of Genes Associated with Gluten Strength,Yellow Pigment Content and PPO Activity in Chinese Wheat Cultivars Journal of Triticeae Crops 2008,28 (2) 220-226

小麦品种面筋强度、黄色素含量和PPO活性相关基因的分子检测

作  者：
杨芳萍;何中虎;杨文雄;尚勋武;夏先春
单  位：
甘肃农业科学院作物研究所;甘肃农业大学农学院;中国农业科学院作物科学研究所/国家小麦改良中心
关键词：
普通小麦;品质基因;分子标记
摘  要：
为给小麦品质育种筛选亲本材料,并进一步验证相关标记的有效性和实用性,利用5个小麦品质性状基因的分子标记[包括高分子量麦谷蛋白亚基(HMW-GS)Dx5和By8标记、1B/1R易位系特异性SCAR标记、位于7AL染色体与黄色素含量相关的八氢番茄红素合成酶(Phytoene synthase,PSY)基因Psy-A1的标记YP7A、位于2AL染色体的PPO活性基因Ppo-A1的标记PPO18]对157份小麦品种和优良新品系进行了基因等位变异检测。结果表明:(1)在所检测的小麦品种(系)中,Dx5、By8、1B/1R易位系、PSY和籽粒PPO活性基因等位变异存在一定差异。其中,含Dx5的材料29份,占18.5%,含By8和1B/1R易位系的材料分别为68和69份,占43.3%和43.9%,含低黄色素含量等位变异基因Psy-A1b的材料42份,占26.8%,含低PPO活性等位变异Ppo-A1b的等位变异材料78份,占49.7%;(2)157份材料中品质性状基因皆符合要求的只有一个品系(37042),聚合多个优良性状的品质改良工作急需加强;(3)本实验使用的标记均为基因特异性标记,重复性好、准确率高,可有效地应用于小麦品质改良的分子标记辅助选择。
译  名：
Molecular Characterization of Genes Associated with Gluten Strength,Yellow Pigment Content and PPO Activity in Chinese Wheat Cultivars
作  者：
YANG Fang-ping1,2,3,HE Zhong-hu2,YANG Wen-xiong3,SHANG Xun-wu1,XIA Xian-chun2(1.College of Agronomy,Gansu Agricultural University,Lanzhou,Gansu 730070,China;2.Institute of Crop Science,National Wheat Improvement Center,Chinese Academy of Agricultural Sciences,Beijing 100081;3.Crop Research Institute,Gansu Academy of Agricultural Sciences,Lanzhou,Gansu 730070,China)
关键词：
Common wheat(Triticum aestivum L.);Quality trait;Molecular marker
摘  要：
Characterization of wheat quality genes is of great importance in quality improvement and molecular breeding.In the present study,5 gene specific markers for HMW-GS QX(Y15Dx5QX)and QX(Y15By8QX),1B/1R translocation,QX(Y15Psy-A1QX) and QX(Y15Ppo-A1QX) genes were used to detect the allelic variations at these loci in 157 Chinese wheat cultivars and advanced lines.The results indicated that different frequencies were detected for the allelic variations of QX(Y15Dx5QX) and QX(Y15By8QX),1B/1R translocation,QX(Y15Psy-A1QX) and QX(Y15Ppo-A1QX) genes in the wheat cultivars and advanced lines tested.The number of cultivars was 29,68,69,42 and 78 with QX(Y15Dx5,By8,1B/1R,Psy-A1bQX) related to low yellow pigment content and QX(Y15Ppo-A1bQX) associated with low PPO activity,and the frequencies accounted for 18.5%,43.3%,43.9%,26.8% and 49.7%,respectively.Cultivars with all desired alleles for the five genes were present only in one genotype.It is vital to combine several quality genes into a cultivar.The markers employed in this study were all gene-specific,with good repeatability and accuracy,and thus could be used as efficient molecular tools for marker-assisted selection(MAS) in wheat quality improvement programs.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46