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当前位置: 首页 > 文章 > 部分新疆小麦材料Lr34/Yr18及矮秆基因分布规律研究 新疆农业科学 2014,51 (2) 197-204
Position: Home > Articles > Molecular Identification and Distribution of the Lr34/Yr18 Gene and Dwarf Gene in Xinjiang Wheat Cultivars Xinjiang Agricultural Sciences 2014,51 (2) 197-204

部分新疆小麦材料Lr34/Yr18及矮秆基因分布规律研究

作  者：
芦静;曹俊梅;黄天荣;梁晓东;周安定;张新忠;李建疆;曾潮武;高永红;吴新元
单  位：
新疆农业科学院粮食作物研究所
关键词：
普通小麦;Lr34/Yr18基因,矮秆基因;Rht-B1b;Rht-D1b;Rht8;分子标记
摘  要：
【目的】明确新疆小麦材料中慢锈基因Lr34/Yr18、矮秆基因Rht-B1b、Rht-D1b及Rht8基因的等位变异组成和分布,以帮助小麦育种者进行慢病性品种选育和株高改良奠定基础。【方法】使用csLV34、NHBF.2与WR1.2、NH-BF.2与MR1、DF与MR2、Xgwm261标记,对新疆小麦材料中慢锈基因Lr34/Yr18和矮秆基因Rht-B1b、Rht-D1b和Rht8的分布情况进行分子标记鉴定。【结果】在鉴定335个品种(系)中,含Lr34/Yr18的材料占总数的9.25%,可见含Lr34/Yr18慢病基因在新疆小麦育种材料中比较匮乏;有129个材料携带Rht-B1b基因,占总数的38.51%。221份携带Rht-D1b基因,占总数的65.97%;检测Rht8基因,有61份材料扩增出192 bp片段,占总数的18.21%。对于矮杆基团,同时扩出Rht-B1b/Rht-D1b/Rht8基因的材料有11份,占3.28%,扩出Rht-B1b/Rht-D1b基因的有70份,占20.9%,扩出Rht-B1b/Rht8基因的材料有8份,占2.39%。扩出Rht-D1b/Rht8基因的材料有22份,占6.57%。新疆育种材料中以Rht-B1b/Rht-D1b基因占主导地位。【结论】csLV34、NH-BF.2与WR1.2、NH-BF.2与MR1、DF与MR2、Xgwm261标记可以方便、快速、准确地检测Lr34/Yr18、Rht-B1b、Rht-D1b与Rht8基因,能作为小麦材料慢锈基因与矮秆基因鉴定的有效工具,直接利用此标记进行标记辅助选择。
译  名：
Molecular Identification and Distribution of the Lr34/Yr18 Gene and Dwarf Gene in Xinjiang Wheat Cultivars
作  者：
LU Jing;CAO Jun-mei;HUANG Tian-rong;LIANG Xiao-dong;ZHOU An-ding;ZHANG Xin-zhong;LI Jian-jiang;ZENG Chao-wu;GAO Yong-hong;WU Xin-yuan;Research Institute of Grain Crops,Xinjiang Academy of Agricultural Sciences;
关键词：
wheat;;Lr34 /Yr18 gene;;dwarf gene;;Rht-B1b;;Rht-D1b;;Rht8;;molecular markers
摘  要：
【Objective】The allelic composition and distribution of slow-rusting gene Lr34 /Yr18 and dwarfing genes Rht-B1b,Rht-D1b and Rht8 in Xinjiang wheat cultivars were studied to help wheat breeders improve rust resistance and plant height.【Method】Using molecular markers csLV34,NH-BF. 2,WR1. 2,NH-BF. 2,MR1,DF,MR2 and Xgwm261 to test the distribution of slow-rusting gene Lr34 / Yr18 and dwarfing genes Rht-B1b,Rht-D1b and Rht8 in Xinjiang wheat cultivars. 【Result】In all 335 varieties( lines),9. 25% of lines were detected to have allele Lr34/Yr18. It showed that slow rusting gene Lr34/Yr18 was lacking in Xinjiang wheat breeding lines; one hundred and twenty-nine lines( 38. 51%) were detected tohave allele Rht-B1b,two hundred and twenty-one lines( 65. 97%) were detected to have allele Rht-D1b; 192 bp fragment was amplified from the sixty-one lines( 18. 21%) in detecting allele Rht8. Eleven lines( 3. 28%) were detected to possess alleles Rht-B1b,Rht-D1b and Rht8. Seventy lines( 20. 9%) were detected to possess alleles Rht-B1b and Rht-D1b. Eight lines( 2. 39%) were detected to possess alleles Rht-B1b and Rht8. Twenty-two lines( 6. 57%) were detected to possess alleles Rht-D1b and Rht8. the Rht-B1b and Rht-D1b genes had a dominant position in Xinjiang breeding lines. 【Conclusion】Using markers csLV34,NH-BF. 2 and WR1. 2,NH-BF. 2 and MR1,DF and MR2,Xgwm 261 could easily,quickly and accurately test Lr34 / Yr18,Rht-B1b,Rht-D1b and Rht8 genes,which could be used as a effective tool to identify slow rusting genes and dwarfing gene of wheat breeding lines for molecular marker-assisted selection.

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