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当前位置: 首页 > 文章 > 氯氰菊酯高效降解菌的筛选鉴定及其降解特性 食品科学 2012,33 (21) 157-163
Position: Home > Articles > Isolation,Identification and Characterization of a Cypermethrin-Degradating Strain FOOD SCIENCE 2012,33 (21) 157-163

氯氰菊酯高效降解菌的筛选鉴定及其降解特性

作  者：
赖文;刘书亮;赵楠;袁怀瑜
单  位：
四川农业大学食品学院
关键词：
氯氰菊酯;生物降解;地衣芽孢杆菌;降解特性
摘  要：
从四川省雅安市茶园土壤中分离到1株能高效降解氯氰菊酯的细菌B-1。根据其形态、生理生化特征和16S rDNA序列分析(登录号HQ009796),将该菌鉴定为Bacillus licheniformis。菌株B-1以共代谢方式降解氯氰菊酯,对20mg/L氯氰菊酯72h降解率达93.93%,对100mg/L氯氰菊酯240h降解率为70.35%,能检测到其降解中间产物3-苯氧基苯甲酸(3-PBA);菌株B-1对20mg/L溴氰菊酯、氟氯氰菊酯和氰戊菊酯72h降解率分别为95.62%、79.73%和55.67%;菌株B-1对氯氰菊酯降解作用源于其所产生降解酯酶,为非诱导酶,存在于细胞内外,其主要分泌于胞外,同工酯酶电泳进一步表明其胞内外均可能有相同活性降解酯酶带;菌株B-1培养液对小白鼠的急性毒性实验证明其属于实际无毒。
译  名：
Isolation,Identification and Characterization of a Cypermethrin-Degradating Strain
作  者：
LAI Wen,LIU Shu-liang,ZHAO Nan,YUAN Huai-yu (College of Food Science,Sichuan Agricultural University,Ya'an 625014,China)
关键词：
cypermethrin;biodegradation;Bacillus licheniformis;degradation characteristics
摘  要：
A cypermethrin-degrading strain named B-1 was isolated from soil samples collected from a tea garden in Ya'an,Sichuan province.According to its morphological,physiological and biochemical characteristics and 16S rDNA sequence(GenBank Accession No.HQ009796),B-1 was identified as Bacillus licheniformis.B-1 degraded cypermethrin by a co-metabolic mechanism and the degradation rate was 93.93% for 20 mg/L cypermethrin in 72 h and 70.35% for 100 mg/L cypermethrin in 240 h.3-PBA was detected as an intermediate product during the degradation process.The degradation rates for deltamethrin,cyfluthrin and fenvalerate at a concentration of 20 mg/L were 95.62%,79.73% and 55.67% in 72 h,respectively.B-1 could produce a non-inducible esterase that degrades cypermethrin.The enzyme was found both inside and outside the cells mainly as an extracellular product.Isozyme electrophoresis analysis suggested that intracellular and extracellular esterase extracts from strain B-1 had the same esterase electrophoretic pattern.Acute toxicity tests showed that culture supernatant of strain B-1 had no observed toxicity for mice.

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