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当前位置: 首页 > 文章 > 地衣芽孢杆菌分批培养降解氯氰菊酯动力学研究 食品科学 2016,37 (17) 139-144
Position: Home > Articles > Kinetic Models for Batch Fermentation of Bacillus licheniformis and Consequent Cypermethrin Degradation FOOD SCIENCE 2016,37 (17) 139-144

地衣芽孢杆菌分批培养降解氯氰菊酯动力学研究

作  者：
张梦梅;胡露;赖文;姚开;刘书亮
单  位：
四川农业大学食品学院;四川大学轻纺与食品学院
关键词：
氯氰菊酯;分批培养;降解;动力学模型
摘  要：
分析10 L发酵罐中地衣芽孢杆菌降解氯氰菊酯的分批培养动力学。以装料系数0.65、体积分数6.25%的接种量、初始pH 7.5、培养温度37℃、转速300 r/min为发酵条件,探讨地衣芽孢杆菌B-1分批培养过程中菌体生长、氯氰菊酯降解酯酶合成、底物消耗及氯氰菊酯降解的规律。在Logistic方程、Luedeking-Piret方程、Luedeking-Piret-Like方程及动力学一级模型的基础上进行非线性拟合,建立了菌株B-1菌体生长、氯氰菊酯降解酯酶生成、基质——葡萄糖消耗和氯氰菊酯降解动力学模型。将实验实测值与模型理论值进行比较,结果表明模型能较好地反映菌株分批培养过程的动力学特征。
译  名：
Kinetic Models for Batch Fermentation of Bacillus licheniformis and Consequent Cypermethrin Degradation
作  者：
ZHANG Mengmei;HU Lu;LAI Wen;YAO Kai;LIU Shuliang;College of Food Science, Sichuan Agricultural University;College of Light Industry, Textile and Food Engineering, Sichuan University;Institute of Food Processing and Safety, Sichuan Agricultural University;
关键词：
cypermethrin;;batch fermentation;;degradation;;kinetic models
摘  要：
The kinetics of the batch fermentation of Bacillus licheniformis for cypermethrin degradation in a 10 L fermentor was analyzed. Cell growth, the production of cypermethrin-degrading esterase, substrate consumption and cypermethrin degradation were investigated when the fermentation conditions were as follows: filling coefficient, 0.65; inoculum amount, 6.25%(V/V); initial pH, 7.5; temperature, 37 ℃; and rotation speed, 300 r/min. Kinetic models for the growth ofBacillus licheniformis B-1, cypermethrin-degrading esterase production, substrate(glucose) consumption and cypermethrin degradation were established through nonlinear regression using Logistic equation, Luedeking-Piret equation, LuedekingPiret-Like equation and first-order kinetic model. The results showed that the predicted values from these models were in good agreement with the experimental data and these models could provide a reasonable description for the batch fermentation process of Bacillus licheniformis B-1 strain.

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