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几丁质固定化壳聚糖酶的研究

作  者:
曾嘉;郑连英
单  位:
浙江大学化工系生物工程研究所
关键词:
几丁质;固定化;壳聚糖酶;壳聚糖
摘  要:
以几丁质为载体,戊二醛为交联剂,固定壳聚糖酶,对壳聚糖酶的固定化条件、固定化酶的性质进行了研究,确定了酶固定的最佳条件为0.1克几丁质与5ml5%戊二醛交联,固定2mg壳聚糖酶,在此条件下酶活力回收可达70%。固定化酶的最适温度和pH分别为60℃和4.0,动力学参数Km值为17.66g/L。将固定化酶于70℃水浴保温150min,酶活力未见明显下降。该固定化酶具有良好的操作和保存稳定性。
译  名:
Study on Optinal Conditions of Immobilized Chifosanase on Chitin
作  者:
Zeng Jia et al.
关键词:
Chitin Immobilization Chitosanase Chitosan
摘  要:
Chitosanase was immobilized on chitin with glutaraldehyde by cross-link reaction,the immobilization conditions and characterization of the immobilized enzyme were studied.The optimal conditions for immobilization were as follows:0.1g chitin was treated with 5ml 5% solution of glutaraldehyde,then 2mg chitosanase was immobilized on the carrier.Optimal temperature and pH for the immobilized enzyme were 60℃ and 4.0 respectively.Kinetic parameter Km was 17.66g/L.The immobilized enzyme showed good temperature stability,and its operation and storage stability were also very good.

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