当前位置: 首页 > 文章 > 转菊芋凝集素基因烟草的抗蚜性研究 河南农业科学 2004 (2) 16-19
Position: Home > Articles > Studies on the Resistance of Tobacco Transformed with Genes of Helianthus tuberosus Agglutinin Against Peach-potato Aphid (Myzus persicae) Journal of Henan Agricultural Sciences 2004 (2) 16-19

转菊芋凝集素基因烟草的抗蚜性研究

作  者:
梁峰;刘秀花;刘茵;闫永锋;常团结
单  位:
商丘师范学院生物系
关键词:
菊芋;凝集素;抗虫性;桃蚜;转基因烟草
摘  要:
研究了转菊芋凝集素基因对桃蚜的抗性。结果表明 ,转基因烟草对桃蚜具有抗性。同对照植株相比 ,T0 转基因烟草使桃蚜的平均虫头数下降 70 %。在T1 转基因烟草中 ,转hta -b和hta -c基因烟草对桃蚜繁殖的抑制率分别为 5 0 .1 %,64.6%,同时 ,对桃蚜若虫的发育具有明显的抑制作用。初步认为 ,菊芋凝集素基因是一种对同翅目害虫具有抗性的新基因 ,对同翅目害虫的抗虫基因工程具有应用价值
译  名:
Studies on the Resistance of Tobacco Transformed with Genes of Helianthus tuberosus Agglutinin Against Peach-potato Aphid (Myzus persicae)
作  者:
LIANG Feng,LIU Xiu hua, LIU Yin, YAN Yong feng, CHANG Tuan jie (Department of Biology, Shangqiu Normal College,Shangqiu 476000,China)
关键词:
Helianthus tuberosus;Agglutinin;Insect resistance;Peach potato aphid;Transgenic tobacco
摘  要:
Bioassay with peach potato aphid (Myzus persicae) demonstrated that transgenic tobacco had resistance to peach potato aphid.Compared with controls the average population of aphid fed with transgenic plants of T 0 generation during an 11 day assay decreased by 70%.In transgenic plants of T 1 generation, aphid fecundity inhibitions were 50.1% (hta-b) and 64.6% (hta-c) respectively. The development of aphids was notably retarded.It is suggested that hta can be a new gene that has resistance to homopteran insectpest and has applied value for plant transgenic engineering against homopteran insect pests.

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