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当前位置: 首页 > 文章 > LPS刺激条件下鸡和鸭脾脏淋巴细胞归巢相关分子的比较研究 畜牧与兽医 2018 (7) 68-74
Position: Home > Articles > A comparative study on the lymphocyte homing related molecules in chicken and duck after LPS stimulation Animal Husbandry & Veterinary Medicine 2018 (7) 68-74

LPS刺激条件下鸡和鸭脾脏淋巴细胞归巢相关分子的比较研究

作  者：
王涛只;孙雪婧;刘恩雪;陈鸿;黄宇飞;陈秋生
单  位：
南京农业大学动物医学院
关键词：
脂多糖;淋巴细胞;归巢;紧密连接;迁移;黏着斑
摘  要：
本研究旨在比较鸡和鸭在脂多糖(LPS)刺激下脾脏淋巴细胞归巢相关分子的差异性表达。腹腔注LPS 3h后,光镜和电镜下鸡和鸭脾脏红髓均可见大量的淋巴细胞和粒细胞,鸭内皮细胞间隙和椭球结构均较疏松,并可见迁移至椭球周围淋巴鞘PELS中的椭球相关细胞。Western blot显示内皮细胞间连接蛋白Claudin-10表达量在鸡脾脏显著升高,在鸭脾脏显著降低(P<0.05);血管黏附蛋白VCAM-1在鸡脾脏表达量显著升高(P<0.05),在鸭脾脏下降不明显。荧光定量PCR显示,LPS注射后淋巴细胞归巢基因MAd CAM-1,VCAM-1和Integrinα4在鸡脾脏中表达量均显著升高(P<0.05),而在鸭脾脏中表达量显著降低(P<0.05);调节细胞运动相关基因RHO-A和FAK在鸡脾脏表达量显著性下降(P<0.05),Cdc42降低不明显,而RHO-A、FAK和Cdc42在鸭脾脏显著升高(P<0.05)。LPS处理后鸡和鸭均产生了宿主免疫反应,并招募大量淋巴细胞和粒细胞参与炎症反应。鸡脾脏淋巴细胞归巢的发生主要归于内皮细胞与淋巴细胞之间的黏附作用;而鸭脾脏淋巴细胞归巢的发生可能归于两方面:一是细胞间调节紧密连接的蛋白表达量下降引起细胞间的渗透性增强,间接地促进了细胞的迁移,二是通过上调细胞迁移和运动方向的相关基因,进而增加淋巴细胞的迁移能力。本研究为鸡和鸭脾脏炎症刺激后淋巴细胞归巢的分子机制比较提供了基础。
译  名：
A comparative study on the lymphocyte homing related molecules in chicken and duck after LPS stimulation
作  者：
WANG Taozhi;SUN Xuejing;LIU Enxue;CHEN Hong;HUANG Yufei;CHEN Qiusheng;College of Veterinary Medicine,Nanjing Agricultural University;
关键词：
LPS;;lymphocyte;;homing;;tight junction;;migration;;focal adhesion
摘  要：
The aim of this study was to compare the different levels of expression of lymphocyte homing related molecules in chicken and duck after LPS stimulation. LPS was intraperitoneally injected into the experimental birds. Three hours later,observation was performed under the light microscope and electron microscope. The results were: a large number of lymphocytes and granulocytes were found in the red pulp of the chicken and duck spleens. The intercellular space of endothelial cells and ellipsoid structure in the duck spleen were relatively loose,and the ellipsoid related cells were seen to have migrated to periellipsoidal lymphocytic sheath( PELS). Western blot analysis showed that the expression of intercellular connexin Claudin-10 proteins was significantly increased in chicken but significantly decreased in duck( P<0. 05).The expression of adhesive protein VCAM-1 increased significantly in chicken spleen( P<0. 05),but decreased in duck spleen. The expression of lymphocyte homing genes,like MAd CAM-1,VCAM-1 and Integrinα4 of the treatment group increased significantly in chicken spleen( P<0. 05),while in duck spleen the expression significantly decreased( P<0. 05). After LPS injection,the expression of cell movement related genes,like RHO-A and FAK in chicken decreased significantly( P<0. 05),but increased significantly in duck spleen. The expression of Cdc42 in chicken spleen decreased insignificantly,but increased significantly in duck( P< 0. 05). Both chicken and duck spleens had a host immune response to LPS challenge,recruiting a large number of lymphocytes and neutrophils into inflammatory reaction. The lymphocyte homing in chicken was mainly attributed to the adhesion between the endothelial cells and lymphocytes,and in duck it might be attributed to two causes: one was that the decrease in tight junction proteins enhanced the permeability between endothelia cells,which indirectly promoted the migration of lymphocyte; and the other was that upregulation of the migration and movement related genes contributed to the ability of lymphocyte to migrate. The results of the present study provide a basis for the comparison of molecular mechanism of lymphocyte homing in chicken and duck spleens under inflammation.

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