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当前位置: 首页 > 文章 > 武隆猪腰枣的组织培养研究 西南大学学报(自然科学版) 2017,39 (5) 37-42
Position: Home > Articles > Tissue Culture of Jujube (Zizyphus jujuba) Mill.cv.Wulongzhuyaozao Journal of Southwest University(Natural Science Edition) 2017,39 (5) 37-42

武隆猪腰枣的组织培养研究

作  者：
夏清清;刘蓝筠;孙海艳;何桥;李晓林;梁国鲁;朱恒星;郭启高
单  位：
南方山地园艺学教育部重点实验室/西南大学;重庆市林业科学院
关键词：
武隆猪腰枣;组织培养;快速繁殖
摘  要：
以武隆猪腰枣为试材,以茎尖、带芽茎段、节间和叶片为外植体,开展了组织培养研究.结果表明,外植体以带芽茎段最好;适宜的初代培养基以1/2MS+1mg/L 6-BA+0.2mg/L NAA,接种的带芽茎段萌芽快,其愈伤组织的不定芽再生率达53.3%;最佳不定芽继代培养基为MS+1mg/L 6-BA+0.2mg/L IBA,增殖系数达3.77;不定芽在1/2MS+0.3mg/L IBA和1/2MS+0.3mg/L IBA+10g/L AC培养基上生根效果较好,生根率达90%~96.67%.
译  名：
Tissue Culture of Jujube (Zizyphus jujuba) Mill.cv.Wulongzhuyaozao
作  者：
XIA Qing-qing;LIU Lan-jun;SUN Hai-yan;HE Qiao;LI Xiao-lin;LIANG Guo-lu;ZHU Heng-xing;GUO Qi-gao;Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education/School of Horticulture and Landscape Architecture,Southwest University;Chongqing Academy of Forestry;
关键词：
jujube(Zizyphus jujuba)Mill.cv.Wulongzhuyaozao;;tissue culture;;rapid propagation
摘  要：
The shoot-tips,mature stem segments with buds,internodes and leaves were used as explants in a study of the tissue culture of jujube(Zizyphus jujuba)Mill.cv.Wulongzhuyaozao.The mature stem segments with buds were shown to be the most suitable explants.In the primary culture,1/2MS+1mg/L 6-BA+0.2mg/L NAA was the best medium,in which the bud sprouted fast,and the rate of adventitious shoot regeneration from the induced calli was 53.3%.The best medium for proliferation was MS+1mg/L 6-BA+0.2mg/L IBA,and the bud multiplication coefficient was as high as 3.77.The adventitious shoots rooted readily on1/2MS+0.3mg/L IBA+10g/L activated carbon,with a rooting percentage of 90%-96.67%.The identification of suitable conditions for tissue culture of Wulongzhuyaozao laid a good foundation for virus elimination,rejuvenation and variety improvement of this jujube cultivar.

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