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Position: Home > Articles > Somatic Embryo Genesis from in Vitro Leaves of Pear Acta Horticulturae Sinica 2003,30 (1) 85-86+126

西洋梨叶片直接再生体细胞胚

作  者:
孙清荣;刘庆忠;赵瑞华
单  位:
山东省果树研究所;山东农业大学生命科学学院
关键词:
梨;西洋梨;叶片;体细胞胚;再生
摘  要:
以西洋梨‘红安久’和‘绿安久’两个品种的无菌苗幼叶为试材 ,研究了不同培养基对叶片分化不定梢及体细胞胚的影响。结果表明 :基本培养基的组成是影响叶片分化为不定梢还是体细胞胚的关键因素 ,植物生长物质的种类和水平是影响体细胞胚再生率高低的重要因素。诱导培养基NN6 9附加TDZ 2mg/L及NAA 0 .1mg/L ,体细胞胚再生率最高 ,绿安久达 5 5 .5 % ,红安久为 4 7.3%。NN6 9附加BA5mg/L及NAA 0 .1mg/L ,不定梢再生率最高 ,绿安久达 10 0 % ,红安久为 85 .7%。
译  名:
Somatic Embryo Genesis from in Vitro Leaves of Pear
作  者:
Sun Qingrong 1, Liu Qingzhong 1, and Zhao Ruihua 2 ( 1Shandong Institute of Pomology, Tai'an 271000, China; 2 College of Life Science, Shandong Agricultural University, Tai'an 271000, China)
关键词:
Pear( Pyrus communis L.); Leaves; In vitro; Somatic embryo; Regeneration
摘  要:
Young leaves in vitro of pear ( Pyrus communis L.) cultivars Red D'Anjou and D'Anjou were selected as materials. Effect of media on shoot and somatic embryo regeneration from leaves was studied. Results showed that the composition of basic media was the main factor affecting shoot regeneration or somatic embryo regeneration from leaves; the kind and concentration of exogenous hormone supplemented were important factor to higher frequency regeneration of somatic embryo. In this study, somatic embryo regeneration rate was the highest in the treatment with medium NN69 supplemented with 2 mg/L TDZ and 0.1 mg/L NAA, D'Anjou was 55.5%, and Red D'Anjou was 47.3%. Shoot regeneration rate was the highest on NN69 supplemented with 5 mg/L BA and 0.1 mg/L NAA, D'Anjou was 100%, Red D'Anjou was 85.7%.

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