Position: Home > Articles > Effect on Cancer Cell Proliferation of Polysaccharide Extracted from Inonotus obliquus Fruit Bodies Using Ultrasound
Acta Edulis Fungi
2007,14
(4)
51-54
超声提取桦褐孔菌子实体多糖及多糖对癌细胞作用的研究
作 者：
张慧丽;杨松;寇巍;辛茹;李玉;刘宏生
单 位：
辽宁大学生命科学院;沈阳军区药品检验所
关键词：
桦褐孔菌子实体多糖;正交实验;超声提取;肝癌细胞;吞噬指数K
摘 要：
采用正交试验优化桦褐孔菌子实体多糖的超声提取工艺条件,并对提取多糖的抗肿瘤活性进行研究。结果表明,桦褐孔菌多糖超声提取的最佳工艺条件为超声时间70min,温度80℃,频率20kHz,加水量12倍水,其多糖在体外对肝癌HEPG2细胞株具有明显的抑制作用,并可诱导肿瘤细胞凋亡,体内实验发现多糖可显著抑制小鼠S180肉瘤的生长,并具有增强免疫作用。
译 名：
Effect on Cancer Cell Proliferation of Polysaccharide Extracted from Inonotus obliquus Fruit Bodies Using Ultrasound
作 者：
ZHANG Huili1,YANG Song2,KOU Wei1,XIN Ru1,LI Yu1,LIU Hongsheng1(1Department of Life Science,Liaoning University,Shenyang,Liaoning 110036,China;2InstituteFor Drug and Instrument Control,PLA Military Command of Shenyang,Shenyang,Liaoning 110026,China)
关键词：
Inonotus obliquus;Polysaccharide;Ultrasound extraction;Cancer cell line
摘 要：
Optimal conditions for extraction of polysaccharide from Inonotus obliquus fruit bodies(IOP)were determined as follows:extraction time,70 min;temperature,80 ℃;ratio of aqueous solvent to fruit body,12∶1;and ultrasound frequency,20 kHz.IOP at a concentration of 1.44 μg/mL inhibited cancer cell growth by~65% as measured using a tetrazolium-based(MTT)assay to evaluate the anti-proliferative effect of the polysaccharide on hepatocarcinoma HEPG2 cells.A 27% and 35% reduction in tumor weight was also observed in S-180 tumor-bearing mice administered low(50 mL/kg·d,8 μg/mL)and high(100 mL/kg·d,16 μg/mL)doses of IOP,respectively.
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