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当前位置: 首页 > 文章 > 龙眼果实ACS和ACO基因克隆及其表达特性 果树学报 2010,27 (1) 39-44
Position: Home > Articles > Isolation and expression pattern of ACS and ACO genes in longan fruit Journal of Fruit Science 2010,27 (1) 39-44

龙眼果实ACS和ACO基因克隆及其表达特性

作  者：
徐士娟;邝健飞;陆旺金;万嗣宝;陈建业
单  位：
华南农业大学;上海大学生命科学学院上海大学计算机工程与科学学院;华南农业大学园艺学院广东省果蔬保鲜重点实验室;华南农业大学园艺系
关键词：
龙眼果实;ACS基因;ACO基因;克隆;表达特性
摘  要：
采用RT-PCR和RACE扩增技术相结合的方法从龙眼果实中分离得到ACS和ACO基因,并分别命名为DlACS1和DlACO1。DlACS1蛋白一共编码486个氨基酸,含有7个保守区域和11个不变的氨基残基;DlACO1蛋白一共编码315个氨基酸,含有7个保守区域和9个不变的氨基残基。在龙眼果实发育过程中,果实乙烯释放量在幼果期出现一个小峰,随后逐渐降低,至发育后期维持在较低水平。Northern分析表明,果皮中DlACS1和DlACO1的表达均在果实快速生长期达到最高;在果肉中,DlACS1只在果肉生长初期表达,DlACO1表达随着果肉的生长缓慢降低,在果实成熟期略有升高。此外,DlACS1和DlACO1表达不具有组织特异性,但在各组织中的表达有明显差异。上述结果为研究非跃变型果实的成熟与乙烯之间的关系奠定了基础。
译  名：
Isolation and expression pattern of ACS and ACO genes in longan fruit
作  者：
XU Shi-juan1,KUANG Jian-fei2,LU Wang-jin1,WAN Si-bao3,CHEN Jian-ye1 (1Guangdong Key Laboratory for Postharvest Science,College of Horticultural Science,South China Agricultural University,Guangzhou,Guangdong 510642 China; 2South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,Guangdong 510650 China; 3College of Life Sciences,Shanghai University,Shanghai 200444 China)
关键词：
Dimocarpus longan; ACS gene; ACO gene; Cloning; Expression
摘  要：
In the present study,ACS and ACO genes from longan termed DlACS1 and DlACO1,were isolated using the combination of RT-PCR and RACE. DlACS1 protein was a polypeptide of 486 amino acids,including 7 conserved regions and 11 conserved amino residues,and DlACO1 protein consisted of 315 amino acids,covering 7 conserved regions and 9 conserved amino residues. Ethylene production exhibited a small ethylene peak at the early stage of fruit development,but decreased slowly thereafter and maintained a very low level at ripening stage. Northern blotting analysis showed that both DlACS1 and DlACO1 genes expressions in the pericarp were strongest at the rapid fruit growth stage,while in the aril,DlACS1 gene expression could be detected only at the early stage of aril development,and DlACO1 gene expression declined gradually with aril growth,but slightly increased at ripening stage. In addition,the expressions of DlACS1 and DlACO1 did not exhibit tissue-specificity,but showing obvious difference in different tissues. These results provide basis for further study-ing the relationship between ethylene and the ripening of non-climacteric fruit.

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