中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 大黄鱼(♀)与鮸(♂)杂交的遗传分析 水产学报 2008,32 (1) 53-59
Position: Home > Articles > Genetic analysis of the intergenus hybridization of Pseudosciaena crocea(♀)× Miichthys miiuy(♂) Journal of Fisheries of China 2008,32 (1) 53-59

大黄鱼(♀)与鮸(♂)杂交的遗传分析

作  者：
王晓清;王志勇;谢中国;詹炜;宁岳
单  位：
湖南农业大学动物科技学院;集美大学水产学院福建省高校水产科学技术与食品安全重点实验室
关键词：
大黄鱼;鮸;杂交;微卫星标记;AFLP标记;遗传分析
摘  要：
用大黄鱼(♀)和鮸(♂)进行属间杂交,获得了56.25%的受精率,45.24%的孵化率和0.65%的鱼苗成活率。对杂交亲本与子代进行了微卫星和AFLP标记的比较分析,结果表明:4个微卫星位点(LYC0003,LYC0006,LYC0007,LYC0008)都可扩增出清晰的亲本差异条带,26个F1个体中均未出现雄鱼特有条带;4对选择性扩增引物(E-ACC/M-CAG,E-AAC/M-CAT,E-AAC/M-CTA,E-AGC/M-CTC)共检出326个AFLP条带,143条母本特异性条带中有132条出现在子代中,94条父本特异性条带中只有18条出现在子代中,子代中另出现了33条非亲条带。F1个体之间的遗传相似系数为0.891,与母本、父本间的遗传相似系数分别为0.853、0.271;F1个体之间的遗传距离为0.116,与母本、父本间的遗传距离分别为0.159、1.307,表明杂交子代与母本大黄鱼之间具有极高的遗传同质性,属异精雌核发育个体。
译  名：
Genetic analysis of the intergenus hybridization of Pseudosciaena crocea(♀)× Miichthys miiuy(♂)
作  者：
WANG Xiao-qing1,2,WANG Zhi-yong1,XIE Zhong-guo2,ZHAN Wei2,NING Yue1 (1.Key Laboratory of Science and Technology for Aquaculture and Food Safety of Fujian Province University,Fisheries College,Jimei University,Xiamen 361021,China;2.College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,China)
关键词：
Pseudosciaena crocea;Miichthys miiuy;cross;genetic analysis;microsatellite;AFLP
摘  要：
Intergenus hybrids of Pseudosciaena crocea(♀)and Miichthys mijuy(♂)was produced in this study.The results showed that the fertilization rate was 56.25%,and the hatching rate was 45.24%;however,the survival rate was only 0.65% at fry stage.The genetic analysis on the relationship between the F1 progenies and their parents were carried out using AFLP and microsatellite markers.Clear parental specific bands were amplified from all the four microsatellite loci(LYC0003,LYC0006,LYC0007,LYC0008),but none of paternal specific bands were found in all the 26 F1 progenies. In the AFLP results,a total of 312 bands was produced with 4 selective primer pairs(E-ACC/M-CAG,E-AAC/M-CAT,E-AAC/M-CTA,E-AGC/M-CTC),of which 132 were maternal specific bands,94 were paternal specific bands,and 32 were nonparental bands.The average genetic similarity among the F1 progenies was 0.891,genetic distance was 0.116.The average genetic distance between the F1 progenies and their mother was 0.159,while it was 1.307 between them and their father.The results suggested that the F1 progenies were allogynogens for high genetic identity between the F1 and the female parent.

相似文章

导出引用
计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46