关键词：
RS4型抗性淀粉;C57BL/6J小鼠;肠绒毛形态;变性梯度凝胶电泳;肠道菌群
摘 要：
目的:探讨交联辛烯基琥珀酸酯化甘薯淀粉(CLOSA-SPS)、柠檬酸乙酰化甘薯淀粉(CAAC-SPS)及羟丙基交联甘薯淀粉(HPCL-SPS)3种RS4型抗性淀粉(RS)对高脂饮食C57BL/6J小鼠肠道微环境的影响作用。方法:72只雄性C57BL/6J小鼠随机分成基础对照组(CL,给予基础饲料)、高脂对照组(HF,给予高脂饲料)、甘薯原淀粉高脂对照组(HF-SPS,给予添加甘薯原淀粉的高脂饲料,150g/kg)及高脂饲料中添加CLOSA-SPS、CAAC-SPS、HPCL-SPS组(HF-CLOSA-SPS、HF-CAAC-SPS、HF-HPCL-SPS,各150g/kg),共6个实验组。连续饲喂12周后处死小鼠,测定其小肠各肠段绒毛高度、隐窝深度并通过PCR-DGGE技术探究这几种RS4型RS对高脂饮食小鼠肠道菌群的影响。结果:与CL组相比,HF组与HF-SPS组C57BL/6J小鼠小肠各肠段的绒毛高度减小,隐窝深度增大,绒腺比减小,且差异显著(P<0.05);而RS4型RS能不同程度使高脂饮食小鼠肠绒毛高度增大,隐窝深度减小,绒腺比增大,其中,HF-CAAC-SPS组及HF-HPCL-SPS组效果优于HF-CLOSA-SPS组,与HF组及HF-SPS组差异均显著(P<0.05)。高脂饮食会改变小鼠的肠道菌群,聚类分析表明HF组与CL组极不相似,前者多样性指数显著小于后者(P<0.05);RS4型RS组的肠道菌群更相似于CL组,且多样性指数均显著大于HF组及HF-SPS组(P<0.05)。结论:CAAC-SPS、HPCL-SPS及CLOSA-SPS这3种RS4型RS能不同程度改善高脂饮食C57BL/6J小鼠肠绒毛形态及肠道菌群,前两者效果优于第三者。
译 名：
Effect of RS4-type Resistant Starch on Intestinal Villus Morphology and Small Intestinal Flora in High-fat Diet-fed C57BL/6J Mice
作 者：
WU Ying-long;WANG Wen-ting;College of Food Science,Sichuan Agricultural University;
关键词：
RS4-type resistant starch;;C57BL/6J mice;;intestinal villus morphology;;denaturing gradient gel electrophoresis;;intestinal flora
摘 要：
This study aimed to examine the effect of cross-linked octenyl succinic anhydride modified sweet potato starch(CLOSA-SPS),citric acid acetylation modified sweet potato starch(CAAC-SPS) and hydroxypropyl cross-linked modified sweet potato starch(HPCL-SPS) as RS4-type resistant starch(RS) on the small intestinal microenvironment of C57BL/6J mice fed high-fat diet.A total of 72 male C57BL/6J mice were randomly divided into six groups: normal control group(CL,fed basal diet),high-fat group(HF,given high-fat diet),high-fat diet containing sweet potato starch group(HF-SPS,150 g/kg),highfat diet containing CLOSA-SPS,CAAC-SPS or HPCL-SPS groups(HF-CLOSA-SPS,HF-CAAC-SPS and HF-HPCL-SPS,respectively 150 g/kg).The mice were sacrificed after 12 weeks to determine the intestinal villus height and crypt depth and to explore the effect of the RS4-type RS on the intestinal flora of C57BL/6J mice fed high-fed diet.Results showed that HF and HF-SPS diets significantly reduced the intestinal villus height and increased crypt depth in mice,which resulted in a decreased ratio of villus height and crypt depth(V/C),compared to the control group.The RS4-type resistant starches were able to increase the intestinal villus height,reduce the crypt depth and thus increase V/C ratio.The effect of the HF-CAACSPS and HF-HPCL-SPS were higher than that of the HF-CLOSA-SPS,which were significantly different(P<0.05) from the HF and HF-SPS.High-fat diet changed the intestinal flora of mice.The UPGMA analysis showed that the HF group and CL group were significantly different and that the diversity index of the former was significantly lower than that of the latter(P<0.05).The intestinal flora of mice fed the RS4-type RSs was quite similar to that of the CL group and the diversity indices were significantly greater than those of the HF group and HF-SPS group(P<0.05).In conclusion,CAAC-SPS,HPCL-SPS and CLOSA-SPS,the 3 kinds of RS4-type RS could improve the morphology of intestinal villus and intestinal flora of C57BL/6J mice fed high-fat diet.
