关键词：
RS4型抗性淀粉;肥胖;炎症因子;脂肪细胞
摘 要：
目的:研究3种RS4型抗性淀粉对高脂饮食C57BL/6J小鼠炎症因子的影响。方法:雄性C57BL/6J小鼠随机分成6组:基础对照(control,CL)组、高脂饮食(high-fat,HF)组及高脂饲料中分别添加15%甘薯淀粉(sweet potato starch,SPS)、羟丙基甘薯淀粉磷酸酯(hydroxypropyl cross-linked sweet-potato modified starch,HPCL-SPS)、交联辛烯基琥珀酸甘薯淀粉酯(cross-linked octenyl succinic anhydride sweet-potato modified starch,CLOSA-SPS)、柠檬酸乙酰化甘薯淀粉酯(citric acid acetylated sweet-potato modified starch,CAAC-SPS)饲喂12周,处死后测定血清中游离脂肪酸(free fatty acid,FFA)、肿瘤坏死因子α(necrosis factor-α,TNF-α)、白介素6(interlukin 6,IL-6)、单核细胞趋化蛋白1(monocyte-chemoattractant protein 1,MCP-1)等指标。结果:HF组和HF-SPS组的FFA、TNF-α、IL-6和MCP-1水平明显高于CL组,HF-HPCL-SPS组、HF-CAAC-SPS组的FFA、TNF-α、IL-6和MCP-1的含量及附睾周围脂肪大小显著低于HF组(P<0.05)。结论:HPCL-SPS、CAAC-SPS可以降低高脂饮食C57BL/6J小鼠血清中FFA和炎症因子水平,并影响脂肪细胞的大小,可能对由肥胖引起的低度炎症及相关代谢疾病有一定的改善作用。
译 名：
Effect of RS4-Type Resistant Starch on Inflammatory Cytokines in C57BL/6J Mice Fed High-Fat Diet
作 者：
WANG Xin;WU Ying-long;College of Food Science,Sichuan Agricultural University;
关键词：
RS4-type resistant starch;;obesity;;inflammatory cytokines;;adipocyte
摘 要：
Objective: To analyze the effects of three kinds of RS4 resistant starch on inflammatory cytokines in C57/BL6J mice fed high-fat diet. Methods: A total of 72 C57BL/6J male mice were randomly divided into six groups with 12 mice in each group: control group(CL), high-fat group(HF) and HF-SPS, HF-HPCL-SPS, HF-CLOSA-SPS and HF-CAAC-SPS groups, fed a high fat diet containing 15% sweet potato starch(SPS), hydroxypropyl cross-linked sweet-potato modified starch(HPCL-SPS), cross-linked octenyl succinic anhydride sweet-potato modified starch(CLOSA-SPS) and citric acid acetylated sweet-potato modified starch(CAAC-SPS), respectively. After 12 weeks of feeding, the levels of serum free fatty acid(FFA), necrosis factor-α(TNF-α), interlukin6(IL-6) and monocyte-chemoattractant protein 1(MCP-1) were determined. Results: The levels of serum FFA, TNF-α, IL-6 and MCP-1 of HF group and HF-SPS group were significantly higher than those in CL group. The size of epididymal adipocyte and the levels of serum FFA, TNF-α, IL-6 and MCP-1 were reduced by HPCL-SPS and CAAC-SPS treatments. Conclusions: HPCL-SPS and CAAC-SPS can suppress the serum F FA and inflammatory cytokines and affect the size of epididymal adipocyte in C57BL/6J mice fed high-fat diet, and thus, have an improvement on obesity-related low-grade inflammatory state and metabolic diseases.
