当前位置: 首页 > 文章 > 米胚芽中α-生育酚的高效液相色谱法测定 浙江大学学报(农业与生命科学版) 1988 (2)
Position: Home > Articles > DETERMINATION OF α-TOCOPHEROL IN RICE-GERM BY REVERSED-PHASE HPLC Journal of Zhejiang University (Agriculture and Life Sciences) 1988 (2)

米胚芽中α-生育酚的高效液相色谱法测定

作  者:
陈忠明;胡军;李家胜
单  位:
浙江农业大学食品科技系
关键词:
米胚芽;α-生育酚;高效液相色谱法;分析
摘  要:
应用反相高效液相色谱法测定了米胚中α-生育酚的含量。样品用无水乙醇索氏法提取后直接加固体氢氧化钾皂化。皂化物经石油醚液液分配、浓缩和转溶于乙醇后,用具C_(18)分离柱—甲醇流动相—292nmUV检测的色谱系统分析,测得一般粗米糠胚中α-生育酚含量为19.94±0.49mg/100g,方法回收率为95±4%,浓度范围在65ng~320ng之间呈线性。
译  名:
DETERMINATION OF α-TOCOPHEROL IN RICE-GERM BY REVERSED-PHASE HPLC
作  者:
Chen Zhongming Hu Jun Li Jiasheng (Department of Food Science and Technology,Zhejiang Agricultural University)
关键词:
rice germ;α-tocopherol;high performance liquid chromatbgraphy;analysis
摘  要:
This paper describes the application of high performance liquid chromato-graphy(HPLC)to the determination of cc-tocopherol in rice germ.The samples were pretreated by the following main steps-the soxhlet extraction with absolute alcohol,saponification.Liquid-Iiuqid distribution with petroleum ether,evaporation and redissolve in alcohol.The alcoholic extracts were analysed by HPLC with C18 column,a mobile phase of 100% methanol and the UV detection of 292nm.The recovery and content of a-tocopherol obtained by using this method are 95±4% and 15.94±0.49mg/100g,respectively.

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