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生防芽孢杆菌8-32对盆栽大豆土壤酶系和微生物区系的影响

作  者:
杨可欣;王欢;刘雪娇;时向哲;贾田惠;高同国
单  位:
河北农业大学生命科学学院
关键词:
大豆根腐病;生物防治;Bacillus subtilis;土壤酶系;微生物区系
摘  要:
前期研究中筛选到一株对大豆根腐病病原菌-尖孢镰刀菌具有较强拮抗作用的菌株Bacillus subtilis 8-32,其生防效果达32. 08%。本试验采用盆栽法,研究接种不同浓度枯草芽孢杆菌8-32(Bacillus subtilis 8-32)后,土壤酶系和微生物区系的变化情况。结果表明:使用枯草芽孢杆菌8-32后土壤脲酶活性增加了16. 9%~66. 7%,蛋白酶活性增加了88. 8%~131. 0%,酸性磷酸酶活性增加了20. 6%~51. 8%,碱性磷酸酶活性增加了7. 5%~14. 6%,根际土壤中细菌数量增加8. 5%~72. 5%,对土壤中真菌数量抑制率最高达37. 2%,放线菌数量也有明显增加。说明菌株8-32对由尖孢镰刀菌引起的大豆根腐病有显著的防治效果,具有进一步应用的潜力。
译  名:
Effect of Bacillus Subtilis 8-32 on Soil Enzymes and Microflora of Potted Soybean
作  者:
YANG Ke-xin;WANG Huan;LIU Xue-jiao;SHI Xiang-zhe;JIA Tian-hui;GAO Tong-guo;College of Life Science,Agricultural University of Hebei;
单  位:
YANG Ke-xin%WANG Huan%LIU Xue-jiao%SHI Xiang-zhe%JIA Tian-hui%GAO Tong-guo%College of Life Science,Agricultural University of Hebei
关键词:
Soybean root rot;;Biocontrol;;Bacillus subtilis;;Soil enzymes;;Microflora
摘  要:
In previous study,a strain Bacillus subtilis 8-32,which had strong antagonistic ability against to Fusarium oxysporum was screened,its biocontrol effect reached to 32. 08%. In this experiment,further study was carried out by pot experiments. We studied the changing of soil enzyme system and microflora after inoculated different concentration of Bacillus subtilis 8-32. The results showed that: Bacillus subtilis 8-32 increased the activity of soil urease by 16. 9%-66. 7%,protease activity increased by 88. 8%-131. 0%,acid phosphatase activity increased by 20. 6%-51. 8%,alkaline phosphatase activity increased by 7. 5%-14. 6% and bacteria in soil increased by 8. 5%-72. 5%,the quantity of soil fungi inhibition rate reached to 37. 2%,the number of actinomyces increased obviously. It indicated that strain 8-32 has significant control effect on soybean root rot caused by Fusarium oxysporum,and has potential for further application.

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