中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 匍匐小菊遗传转化过程中选择压力的确定 北方园艺 2008 (10) 147-149
Position: Home > Articles > Determination of Selection Pressure on Genetic Transformation of Dendranthema morifolium cv.pink carpet Northern Horticulture 2008 (10) 147-149

匍匐小菊遗传转化过程中选择压力的确定

作  者：
高凤娟;黄丛林;马荣才;张秀海
单  位：
首都师范大学
关键词：
菊花;叶盘;再生体系;卡那霉素;草丁膦
摘  要：
该试验以北京农科院生物中心新近培育的匍匐小菊品种"粉地毯"为材料,以叶片为外植体,建立"粉匍"的高效再生体系。试验共进行了6种不同培养基的筛选,研究了6-BA对于再生频率的影响,最终确立了6-BA的最佳浓度。在MS+6-BA(0.5 mg/L)+NAA(0.1 mg/L)培养基上获得91%的再生率,在1/2 MS+NAA(0.1 mg/L)培养基上诱导生根。在获得高效叶盘再生体系的基础上,又研究了植物遗传转化过程中常用的2种选择标记卡那霉素(Km)和除草剂(PPT)对菊花不定芽分化的影响。结果表明:大于8 mg/L的Km和大于0.4 mg/L的PPT能够完全抑制试验材料的不定芽分化。试验最终确定了匍匐小菊对卡那霉素、草丁膦的适宜筛选浓度,分别为5 mg/L和0.2 mg/L,为以后进行匍匐小菊的基因工程工作奠定了基础。
译  名：
Determination of Selection Pressure on Genetic Transformation of Dendranthema morifolium cv.pink carpet
作  者：
GAO Feng-juan1,2,HUANG Cong-lin1,MA Rong-cai1,ZHANG Xiu-hai1(1.Capital Normal University,Beijing 100097,China;2.Beijing Agro-Biotechnology Research Center,Beijing 100097,China)
关键词：
Dendranthema morifolium;Leaf discs;Regeneration system;Kanamycin;Phosphinothriein
摘  要：
High regeneration efficiency system of sprawling Dendranthema morifolium new cultivar"pink carpet" bred recently by Beijing Agro-Biotechnology Research Center was set up with leaf discs as explants.Six different medium were tried to investigate the effects of 6-BA on the Dendranthema regeneration frequency.Proper concentration of 6-BA was determined.Shoots regenernated from 91% of the total explants in the medium MS+6-BA(0.5 mg/L)+NAA(0.1 mg/L).Roots were induced in the medium 1/2 MS+ NAA(0.1 mg/L).After the acquirement of high regeneration frequency system with leaf discs,effects of two commonly used selecting agents in plant genetic transformation,kanamycin(Km)and phosphinothricin(PPT),on the differentiation of adventitious shoots from in vitro leaf discs of Dendranthema morifolium were investigated.The results indicated both Km and PPT completely inhibited shoot induction when Km concentration was higher than 8 mg/L or PPT concentration was higher than 0.4 mg/L.In this paper proper concentration of Km and PPT for selection pressure of genetic transformation was determined as 5 mg/L and 0.2 mg/L individually.This research established a foundation for genetic engineering of sprawling Dendranthema morifolium.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46