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Position: Home > Articles > Mutagenesis Breeding of Bacillus subtilis 515 Strain for Chitinase Production and Study on Its Fermentation Condition Chinese Agricultural Science Bulletin 2010,26 (11) 279-283

高产几丁质酶的枯草芽孢杆菌诱变育种及发酵条件研究

作  者:
张敏;胡晓;万津瑜;雷欣雨;赵丹萍
单  位:
四川农业大学植物病理系
关键词:
几丁质酶;诱变;发酵条件;枯草芽孢杆菌
摘  要:
对从自然界筛选获得的枯草芽孢杆菌Bacillus subtilis515菌株进行紫外线、氯化锂、硫酸二乙酯的复合诱变,获得了1株遗传稳定的高产几丁质酶活性菌株。采用正交试验设计对变异菌株的培养基和培养条件进行优化,采用其优化条件,使变异菌株单位发酵液的几丁质酶活性提高到了1.53U/mL。
译  名:
Mutagenesis Breeding of Bacillus subtilis 515 Strain for Chitinase Production and Study on Its Fermentation Condition
作  者:
Zhang Min, Hu Xiao, Wang Jinyu, Lei Xinyu, Zhao Danping (Department of Plant Pathology, Sichuan Agicultural Universty, Ya'an Sichuan 625015)
关键词:
chitinase; mutagenesis; fermentation condition;Bacillus subtilis 515
摘  要:
A higher chitinase production mutant with stable heritage, was obtained from initialBacillus subtilis 515 strain, which was separated from soil and by means of UV,LiCl and DES mutagenization. The optimum medium and fermentation condition forBacillus subtilis 515 have been established through orthogonal test design. Under the established optimal culture conditions and in the established optimal medium, the chitinase activity ofBacillus subtilis 515 increased to 0.53 U/mL compared with that the initial strain under the culture condition before optimization.

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