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当前位置: 首页 > 文章 > 白腐菌Trametes pubescens MB89产漆酶发酵条件的优化及其部分酶学性质的研究 西北农业学报 2009,18 (4) 175-180
Position: Home > Articles > Optimization of Cultivation Conditions and Properties of Laccase from White Rot Fungus Trametes pubescens MB89 Acta Agriculturae Boreali-occidentalis Sinica 2009,18 (4) 175-180

白腐菌Trametes pubescens MB89产漆酶发酵条件的优化及其部分酶学性质的研究

作  者：
赵凤霞;张莉
单  位：
西北农林科技大学农学院
关键词：
白腐菌;漆酶;酶活性
摘  要：
以白腐菌T. pubescensMB89为漆酶培养菌,研究培养方式、温度和Cu2+等因素对发酵产生漆酶的影响。在漆酶性质研究中,研究了温度、pH值、底物种类、金属离子等因素对漆酶催化活性的影响。结果表明,T. pubescensMB89产漆酶的最佳培养温度为25℃,最适Cu2+浓度1.0μmol/L,120 r/min振荡培养优于静止培养。T. pubescensMB89漆酶氧化底物的最适作用温度为25℃,最适pH随底物的不同而不同。Cu2+、Co2+、K+、Ag+对漆酶活性有促进作用,Fe2+、Fe3+、Ca2+等离子对漆酶活性有抑制作用。Na+、Mg2+、Zn2+、Mn2+等离子对漆酶酶活性影响不大。由此说明,振荡培养T. pubescensMB89生产漆酶的过程中Cu2+的存在可以加速漆酶的产生。T. pubescensMB89漆酶酶活性受温度、pH值、金属离子、底物等因素的影响。
译  名：
Optimization of Cultivation Conditions and Properties of Laccase from White Rot Fungus Trametes pubescens MB89
作  者：
ZHAO Fengxia and ZHANG Li (College of Agronomy,Northwest A&F University,Yangling Shaanxi 712100,China)
关键词：
White fungi;Laccase;Enzyme activity
摘  要：
The optimal fermentation conditions for laccase production by white-rot fungi Trametes pubescens MB89 were studied and laccase properties were analyzed.Culture methods,temperature and copper ions concentration were selected for fermentation conditions optima.Laccase properties were determined by studying the change of enzymatic activity.The result showed that the optimal temperature for laccase production was 25℃,copper ions concentration was 1.0 μmol / L.It was better to shaking the flasks with rate 120 r/min during fermentation.The optimum temperature for laccase activity were 30℃.Optimum pH value was varied with different sbustrates.Various metal ions showed different effects on the activity.The activity was enhanced by Cu2+,Co2+,K+ and Ag+,and was strongly inhibited by Fe2+,Fe3+and Ca2+.It suggested that glucose and peptone could be used as carbon and nitrogen for laccase production by T.pubescens MB89.The fermentation process of the existence of copper can accelerate the emergence of laccase.Substrates,temperature,pH,metal ions could influence the activity of laccase.

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