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当前位置: 首页 > 文章 > 猪伪狂犬病病毒流行毒株gC基因的分子特征及其对小鼠的毒力试验 中国兽医科学 2017 (6) 713-720
Position: Home > Articles > Molecular characterization of gC gene of pseudorabies virus epidemic isolates and their virulence test in mice Chinese Veterinary Science 2017 (6) 713-720

猪伪狂犬病病毒流行毒株gC基因的分子特征及其对小鼠的毒力试验

作  者：
夏德利;黄立平;危艳武;王一平;杜文娟;谢永兴;吴洪丽;冯力;刘长明
单  位：
中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室
关键词：
伪狂犬病病毒;流行毒株;gC基因分子特征;毒力
摘  要：
为了阐明猪伪狂犬病病毒(PRV)流行毒株gC基因的分子特征及其对小鼠的毒力,对来自不同年代分离的6株PRV流行毒株进行了gC基因分子变异特征分析,通过接种BALB/c小鼠检验毒株的毒力。对临床病例分离的6株PRV和30个Gen Bank下载的gC基因序列进行分析表明,这6株PRV与2012年以后流行的毒力增强的变异毒株处于同一分支;这些毒株的氨基酸同源性为98.7%~100%,而与经典的PRV-SC毒株的氨基酸同源性仅为93.2%~94.0%。PRV毒株g C蛋白第52~70位氨基酸为高变区,尤其第64~70位出现7个氨基酸(AAASTPA)插入。对gC蛋白糖基化位点分析表明,PRV变异毒株较经典毒株增加了6个O-连接糖基化位点,且gC蛋白B细胞表位出现3处突变,分别为P69R、P80Q和N83G。gC蛋白上硫酸乙酰肝素结合域(HBD)出现2处突变,分别为HBD1第5位氨基酸由经典毒株的P突变为Q,HBD3的第3位氨基酸由Y突变为C。用分离的6株PRV接种BALB/c小鼠试验显示,PRV-HRB和PRV-SH毒株半数致死量(LD50)均为1×10~(3.21)/mL,PRV-DL、PRV-JL及PRV-GZL的LD50为1×10~(3.75)/mL,PRV-JF的LD50为1×10~(4.32)/mL。上述结果阐明了PRV流行毒株g C基因的分子特征及其对小鼠的毒力,为该病毒的致病机制研究提供了科学依据。
译  名：
Molecular characterization of gC gene of pseudorabies virus epidemic isolates and their virulence test in mice
作  者：
XIA De-li;HUANG Li-ping;WEI Yan-wu;WANG Yi-ping;DU Wen-juan;XIE Yong-xing;WU Hong-li;FENG Li;LIU Chang-ming;State Key Laboratory of Veterinary Biotechnology/Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences;
关键词：
pseudorabies virus;;epidemic isolates;;molecular characterization of the gC gene;;virulence
摘  要：
To elucidate the molecular characterization of the g C gene of pseudorabies virus(PRV)epidemic isolates and their virulence in mice,six PRV isolates from clinical cases at different areas were isolated to be used for the genetic variation analysis and virulence test in BALB/c mice.The g C amino acid sequences of the six PRV isolates and other thirty sequences of PRV strains available in Gen Bank were used for analysis and comparison.The results suggested that the six isolates and PRV variants isolated in China since 2011 belonged to the same branch,and shared from 98.7% to 100%identity with PRV variants,but only from 93.2% to 94.0% identity with the classic strain PRV-SC.A hypervariable region located in No.52—70 aa was found on the g C protein for the six PRV isolates,especially the insert sequences of seven amino acids(64AAASTPA70).The analysis of the g C protein glycosylation sites showed that six O-linked glycosylation sites were added compared with the classic strain PRV-SC.Furthermore,one mutation of P69 R and two mutations of P80 Q and N83 G were found in the B cell epitopes of g C,respectively.Meanwhile,two mutations were found in the heparin binding domain(HBD),of which the fifth amino acid was mutated into Q,and the third amino acid was mutated into C.BALB/c mice were inoculated with the six PRV isolates to evaluate their virulence.The results indicated that the LD50 of PRV-HRB and PRV-SH isolates was 1×10~(3.21)/mL,PRV-DL,PRV-JL and PRV-GZL were 1×10~(3.75)/mL in LD50,while PRV-JF was 1×10~(4.32)/mL in LD50.This study result illuminated the correlation between the molecular characterization of the g C gene of PRV new epidemic isolates and their virulence in mice,which provided a scientific basis for studying the pathogenesis of the virus.

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