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当前位置: 首页 > 文章 > 北京3株H9N2亚型禽流感病毒HA基因的序列分析 安徽农业科学 2009,37 (10) 88-90
Position: Home > Articles > Sequence Analysis of HA Gene from Three H9N2 Avian Influenza Viruses Journal of Anhui Agricultural Sciences 2009,37 (10) 88-90

北京3株H9N2亚型禽流感病毒HA基因的序列分析

作  者：
韩春华;林健;刘月焕;潘洁;马明;刘永宏
单  位：
北京市农林科学院畜牧兽医研究所;内蒙古农业大学;中国农业大学动物医学院
关键词：
H9N2亚型禽流感病毒;HA基因;序列分析
摘  要：
[目的]为了解2000年北京地区H9N2分离毒株的遗传特点。[方法]采用RT-PCR技术扩增了3株H9N2亚型禽流感病毒北京地区分离株的HA基因片段,并对所得序列进行分析比较。[结果]遗传演化分析表明,所分离的3个毒株的HA基因与其他中国内地和香港毒株的同源性分别为XU株84.8%(Dk/HK/Y439/97)~98%(Ck/GX17/00),LIU株85.1%(Dk/HK/Y439/97)~99.1%(Ck/GX17/00),BEI株90.7%(Ck/BJ/3/01)~99.1%(Ck/GX17/00)。氨基酸序列分析发现,3株病毒均为禽源低致病力毒株,226位氨基酸均为L(Leu),更易于与人类细胞结合;HA蛋白存在7个糖基化位点。[结论]从分子水平分析,A/Chicken/Beijing/xu/00、A/Chicken/Beijing/bei/00和A/Chicken/Beijing/liu/00株属于低致病力的H9N2亚型禽源毒株。
译  名：
Sequence Analysis of HA Gene from Three H9N2 Avian Influenza Viruses
作  者：
HAN Chun-hua et al(Institute of Animal Husbandry and Veterinary Medicine,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097)
关键词：
H9N2 subtype avian influenza virus;HA gene;Sequence analysis
摘  要：
[Objective] The study aimed to understand the genetic characters of H9N2 subtype avian influenza viruses isolated in Beijing area.[Method] HA genes of three H9N2 subtype avian influenza viruses A/Chicken/Beijing/xu/00,A/Chicken/Beijing/bei/00 and A/Chicken/Beijing/liu/00 were amplified by RT-PCR and then sequenced.[Result] The results of phylogenetic analysis showed that A/Chicken/Beijing/xu/00,A/Chicken/Beijing/bei/00 and A/Chicken/Beijing/liu/00 shared the nucleotide homology of 84.8%(Dk/HK/Y439/97)-98%(Ck/GX17/00),85.1%(Dk/HK/Y439/97)-99.1%(Ck/GX17/00),90.7%(Ck/BJ/3/01)-99.1%(Ck/GX17/00)with the isolates from Hongkong and other areas of Chinese mainland,respectively.At the same time,the analysis of amino acid indicated that the three isolates belonged to low pathogenic H9N2 isolates of avian origin.The 226th amino acid of them were L(Leu),suggesting high binding affinity to human cells.There were seven glycosylation sites in HA protein,five from HA1 and two from HA2.[Conclusion] By analysis at molecular level,it could be concluded that A/Chicken/Beijing/xu/00,A/Chicken/Beijing/bei/00 and A/Chicken/Beijing/liu/00 were low pathogenic H9N2 isolates of avian origin.

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