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Position: Home > Articles > Isolation and Identification of One Strain Infectious Bursal Disease Virus China Animal Husbandry & Veterinary Medicine 2012,39 (6) 207-211

一株鸡传染性法氏囊病病毒的分离鉴定

作  者:
赵云英;张燕欣;张文生;李富桂
单  位:
天津农学院动物科学系;天津职业大学生物与环境工程学院
关键词:
IBDV;超强毒株;分子鉴定
摘  要:
从天津地区免疫失败的鸡群中分离一株IBDV(命名TJ-hg),应用血清学AGP试验结果可见明显的白色沉淀线;应用逆转录酶—聚合酶链式反应(RT-PCR)扩增的IBDVVP2基因高变区(vVP2)及其序列分析的结果表明,TJ-hg株与超强毒株的核苷酸同源性均在90%以上,氨基酸同源性达98.8%~99.3%,且高变区中特征性氨基酸的变化与超强毒株完全一致,TJ-hg株在第一亲水区氨基酸Q219P发生突变。研究结果表明,TJ-hg株属于超强毒株,但又与标准毒株存在差异。
译  名:
Isolation and Identification of One Strain Infectious Bursal Disease Virus
作  者:
ZHAO Yun-ying 1,ZHANG Yan-xin 1,ZHANG Wen-sheng 2,LI Fu-gui 1(1.Department of Animal Science,Tianjin Agricultural University,Tianjin 300384,China;2.College of Biology and Environmental Engineering,Tianjin Vocational Institute,Tianjin 300402,China)
关键词:
IBDV;very virulent strain;molecular identification
摘  要:
One isolate of infectious bursal disease virus(IBDV),was isolated from the vaccination failure chicken.Through serologic AGP experiment,results showed obvious white precipitation line;the high variable(hv)region of VP2genes were amplified by RT-PCR(reverse transcriptase polymerase chain reaction)and were compared for their sequences.It was indicated that the homology of TJ-hg strain and very virulent IBDV strain all reached above 90%and 98.8%to 99.3%at nucleotide level,respectively.And the change of amino acid was complement in high variable(hv)region of VP2genes.The mutations amino acid Q219Plocated in the first hydrophilic region of TJ-hg strain.The research demonstrated that TJ-hg strain belonged to vvIBDV,but it was different from standard strain.

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