中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 链霉素残留检测ELISA试剂盒的研制及应用 畜牧兽医学报 2008,39 (3) 380-384
Position: Home > Articles > Development and Application of Competitive ELISA Kit for Rapid Detection of Streptomycin Residues Chinese Journal of Animal and Veterinary Sciences 2008,39 (3) 380-384

链霉素残留检测ELISA试剂盒的研制及应用

作  者：
范国英;王顺岗;王自良;张改平;邓润广;杨艳艳
单  位：
河南科技学院动物科学学院;河南省农业科学院免疫学重点实验室
关键词：
链霉素;单克隆抗体;细胞融合;竞争ELISA;快速检测试剂盒
摘  要：
本研究采用EDC法人工合成链霉素抗原(SM-BSA),免疫BALB/c小鼠,通过杂交瘤技术获得了分泌抗链霉素单克隆抗体的杂交瘤细胞株,建立了快速检测链霉素(SM)残留的酶联免疫方法,并成功研制了试剂盒。然后对其灵敏度、准确度、特异性、基质效应性等技术指标进行检测,同时应用该试剂盒对生长猪尿液中的残留情况进行检测。结果表明:除双氢链霉素外,该试剂盒与其它抗生素类药物均无交叉反应;线性检测范围为1~128μg/L,相关系数R2=0.925 1,灵敏度为0.45μg/L,半数抑制浓度(IC50)为7.76μg/L,检测限为1μg/L;猪尿样的平均添加回收率为84.1%;基质对该试剂盒的检测结果影响不大。该试剂盒具有快速、敏感、特异、简便等特点,适合于SM残留的快速检测,具有较高的推广应用价值。
译  名：
Development and Application of Competitive ELISA Kit for Rapid Detection of Streptomycin Residues
作  者：
FAN Guo-ying1,WANG Shun-gang1,WANG Zi-liang1,ZHANG Gai-ping2*,DENG Run-guang2,YANG Yan-yan2(1.College of Animal Science,Henan Institute of Science and Technology,Xinxiang 453003,China;2.Key Laboratory for Animal Immunology,Henan Academy of Agricultural Science,Zhengzhou 450002,China)
关键词：
streptomycin;monoclonal antibody;cell fusion;competitive ELISA;rapid test kit
摘  要：
BALB/c mice were immunized with streptomycin-bovine serum albumin and hybridoma lines that secrete monoclonal antibody against streptomycin(SM mAb) were filtered with cell fusion.A competitive ELISA test kit for detection of streptomycin(SM-Kit)was developed with anti-SM monoclonal antibody(SM mAb) and its sensitivity,veracity,specificity and the effect of matrix were tested,respectively.At the same time,the urine of the swine was detected with this ELISA-Kit.The results indicated that the SM-Kit has little or no cross-reactivity with other antibiotics besides of dihydrostreptomycin;the linear detection was 1.0 to 128.0 μg/L(R2=0.925 1),the sensitivity was 0.45 μg/L,the IC50 was 7.76 μg/L and the detection limit was 1 μg/L.The recoveries of SM spiked in pig urine was 84.1%.The matrix has no effect on the results of the SM-Kit.The SM-Kit possessed rapidity,sensitivity,specificity and briefness and was proved useful in the rapid test of SM residues in animal.

相似文章

导出引用
计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46