中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 4种石斛水提物对人宫颈癌HelaS3细胞和肝癌HepG2细胞的抑制作用 安徽农业科学 2008 (36) 15968-15970
Position: Home > Articles > Inhibitory Effects of Water Extracts from Four Species of Dendrobiums on HelaS3 Cells and HepG2 Cells Journal of Anhui Agricultural Sciences 2008 (36) 15968-15970

4种石斛水提物对人宫颈癌HelaS3细胞和肝癌HepG2细胞的抑制作用

作  者：
鲍丽娟;王军辉;罗建平
单  位：
合肥工业大学生物与食品工程学院
关键词：
霍山石斛;铁皮石斛;金钗石斛;马鞭石斛;肿瘤细胞;抑制作用
摘  要：
[目的]为进一步开发石斛药用资源提供基础数据。[方法]将人宫颈癌细胞HelaS3和肝癌HepG2细胞用含10%新生小牛血清的RPMI-1640培养基在37℃和5%CO2条件下培养,比较了霍山石斛、铁皮石斛、金钗石斛和马鞭石斛水提物对HelaS3和HepG2细胞的体外抑制作用。[结果]4种石斛水提物对HelaS3细胞和HepG2细胞均有不同程度的抑制作用,且在所选浓度范围内对剂量和时间有依赖性。4种石斛水提物对HelaS3细胞的IC50分别是11.63、17.71、15.11和5.89 mg/ml;对HepG2细胞的IC50分别是10.40、22.95、3.80和17.76 mg/ml。显微观察显示,处理组的活细胞多呈红色,核呈固缩状或圆珠状,有明显的染色质凝集和细胞凋亡特征。[结论]剂量效应分析表明,对HelaS3细胞而言,马鞭石斛水提物抑瘤效果较好;对HepG2细胞而言,金钗石斛水提物抑瘤效果较好。
译  名：
Inhibitory Effects of Water Extracts from Four Species of Dendrobiums on HelaS3 Cells and HepG2 Cells
作  者：
BAO Li-juan et al(School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei,Anhui 230009)
关键词：
Dendrobium huoshanense;D.candidum;D.nobile;D.fimbriatum;Tumor cells;Inhibitory effects
摘  要：
[Objective] The study was to provide the foundation data for further development of Dendrobium medicinal resources.[Method] HelaS3 cells and HepG2 cells were put on the RPMI-1640 medium with 10% new newborn calf serum and cultured under 37 ℃ and 5% CO2 to compare in vitro inhibitory effects of water extracts from Dendrobium huoshanense,D.candidum,D.nobile and D.fimbriatum on HelaS3 cells and HepG2 cells.[Result] The water extracts from 4 species of Dendrobium could inhibit the growth of HelaS3 cells and HepG2 cells to different extent,with the inhibitory effects being dose-dependent and time-dependent in the tested concn.range.The IC50 of 4 species of Dendrobium on HelaS3 cells were 11.63,17.71,15.11 and 5.89 mg/ml,resp.and that on on HepG2 cells were 10.40,22.95,3.80 and 17.76 mg/ml,resp.The microscope observations showed that most of the living cells treated with extracts appeared red,with pycnosis of nucleu,agglutination of chromatin and occurrence of apoptosis induced by extracts.[Conclusion] The dose effect analysis showed that the tumor inhibiting effect of water extracts from D.fimbriatum on HelaS3 cells was better and that from D.nobile HepG2 was better.

相似文章

导出引用
计量
文章访问数: 15
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46