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当前位置: 首页 > 文章 > 乳酸锌对猪空肠上皮细胞IPEC-J2增殖及相关调控基因mRNA表达的影响 畜牧兽医学报 2012,43 (5) 740-747
Position: Home > Articles > Effect of Zinc Lactate on Cell Proliferation and Related-regulatory Genes mRNA Expression in Porcine Jejunal Epithelial Cells IPEC-J2 Chinese Journal of Animal and Veterinary Sciences 2012,43 (5) 740-747

乳酸锌对猪空肠上皮细胞IPEC-J2增殖及相关调控基因mRNA表达的影响

作  者：
韩国全;余冰;陈代文;相振田;亓宏伟;陈洪;毛倩;毛湘冰;黄志清
单  位：
四川农业大学动物营养研究所
关键词：
乳酸锌;猪空肠上皮细胞IPEC-J2;mRNA表达;相关调控基因
摘  要：
旨在探讨乳酸锌对猪空肠上皮细胞增殖及相关调控基因ZnT2、DMT1、IREG-1、MT1和ZIP4mRNA表达的影响。用乳酸锌的锌浓度分别为50、100、150、200mg.L-1的培养基培养IPEC-J2细胞,采用比色法测定分析细胞增殖变化;用实时荧光定量RT-PCR方法检测ZnT2、DMT1、IREG-1、MT1及ZIP4mRNA表达,以TBPmRNA的表达水平作为内参对照。在细胞培养前36h,乳酸锌对IPEC-J2细胞基本没有影响,随锌浓度递增细胞增殖幅度升高;乳酸锌处理IPEC-J2细胞后,ZnT2、DMT1、IREG-1及MT1mRNA表达随锌浓度增高而升高,ZIP4mRNA表达则随锌浓度增高而降低。添加乳酸锌可以促进IPEC-J2细胞增殖,上调ZnT2、DMT1、IREG-1、MT1mRNA表达,下调ZIP4mRNA表达。
译  名：
Effect of Zinc Lactate on Cell Proliferation and Related-regulatory Genes mRNA Expression in Porcine Jejunal Epithelial Cells IPEC-J2
作  者：
HAN Guo-quan1,YU Bing1,2,CHEN Dai-wen1,2,XIANG Zhen-tian1,QI Hong-wei1, CHEN Hong1,MAO Qian1,MAO Xiang-bing1,HUANG Zhi-qing1 (1.Institue of Animal Nutrition,Sichuan Agricultural University,Ya'an 625014,China; 2.Key Laboratory of Animal Disease-resistant Nutrition of Ministry of Education,Sichuan Agricultural University,Ya'an 625014,China)
关键词：
zinc lactate;porcine jejunal epithelial cells IPEC-J2;mRNA expression;related-regulatory genes
摘  要：
The present study was conducted to evaluate the effects of zinc lactate on cell proliferation of jejunal epithelial cells IPEC-J2 and mRNA expression of related-regulatory genes in porcine.IPEC-J2 cells were cultured with mediums added with zinc lactate by the zinc concentrations of 50,100,150 and 200 mg·L-1,respectively.Cell proliferation was estimated by Colorimetric analysis.Simultaneously,real-time quantitative RT-PCR was applied to detect the mRNA expression of related-regulatory genes(ZnT2,DMT1,IREG-1,MT1 and ZIP4),and TBP mRNA level was used as the control.During the first 36 hours after treatment with zinc lactate,there was almost no effect on IPEC-J2 cell proliferation.Subsequently,with increasing of the concentration of zinc,the rate of cell proliferation gradually increased.At the same time,after IPEC-J2 cell was treated with zinc lactate,the expression of ZnT2,DMT1,IREG-1and MT1 mRNA was increased with the increase of the concentration of zinc while the expression of ZIP4 mRNA decreased.Zinc lactate could promote cell proliferation of IPEC-J2,up-regulated the mRNA expression of ZnT2,DMT1,IREG-1,MT1 and down-regulated the mRNA expression of ZIP4.

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