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Position: Home > Articles > A p25/Hinf1 RFLP analysis of the Citrus tristeza virus isolates infecting pummelo Plant Protection 2005,31 (4)

柑橘衰退病毒柚分离株的p25/Hinf1 RFLP分析

作  者:
宋震;徐小峰;杨方云;周常勇
单  位:
中国农业科学研究院柑橘研究所
关键词:
植物病毒学;柑橘衰退病毒;分离株;限制性片段长度多态性分析
摘  要:
对123个柑橘衰退病毒柚分离株进行p25/Hinf1 RFLP分析明确:样品中,单一p25/Hinf1 RFLP组群样品占样品总量的82.9%,说明我国田间受侵染柚类中柑橘衰退病毒株系相对较为单一;RFLP组群6的样品占样品总量的79.7%,说明目前柚类生产上的优势流行株属于p25/Hinf1 RFLP组群6,初步认定为强毒株系;柑橘衰退病毒株柚分离株S102、S104、S106、S108属于p25/Hinf1 RFLP组群5,初步认定是弱毒株系。
译  名:
A p25/Hinf1 RFLP analysis of the Citrus tristeza virus isolates infecting pummelo
作  者:
SONG Zhen,XU Xiao-feng,YANG Fang-yun,ZHOU Chang-yong(Citrus Research Institute of Chinese Academy of Agricultural Science,Chongqing 400712,China)
关键词:
phytovirology;Citrus Tristeza Virus;isolate;RFLP assay
摘  要:
Citrus Tristeza Virus(CTV) is one of the most economically important pathogen of citrus in many citrus producing areas of the world.Pummelo dwarf caused by CTV has become a serious problem in China recently.In order to discover their pathogenic characteristics,123 CTV isolates infecting pummelo were studied by p25/Hinf1 RFLP assay.Single p25/Hinf1 RFLP groups were detected in 82.9% of the 123 samples,and the dominant group was p25/Hinf1 RFLP group 6,which accounted for 79.7% of the total.There were 4 isolates belonging to p25/Hinf1 RFLP group 5,which could be mild CTV strains and should be further studied.

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