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嘧肽霉素对烟草花叶病毒抑制作用研究

作  者:
朱春玉;吴元华;王春梅;赵秀香;王艳红;杜春梅
单  位:
黑龙江大学生命学院;沈阳农业大学植物保护学院
关键词:
植物病毒学;嘧肽霉素;TMV;钝化作用
摘  要:
嘧肽霉素和烟草花叶病毒(TMV)等体积混合后接种,对心叶烟的枯斑抑制率为96.5%,对系统寄主NC89的防效为84.9%。在接种病毒前24 h喷施药剂,枯斑抑制率和对系统寄主的防效分别为75.4%和69.5%。接种病毒后24 h喷药试验表明,药剂能在一定程度上抑制进入植株体内病毒的增殖。药剂与病毒混合处理后电镜观察,病毒粒子有断裂现象,混合时间越长,破坏作用越强。嘧肽霉素对TMV具有较强的体外钝化作用,且是不可逆的。
译  名:
Inhibition of cytosinpeptidemycin on Tobacco Mosaic Virus
作  者:
ZHU Chun-yu~1,WU Yuan-hua~1,WANG Chun-mei~1,ZHAO Xiu-xiang~1, WANG Yan-hong~1,DU Chun-mei~2(1.College of Plant Protection,Shenyang Agricultural University,Shenyang 110161,China;2.College of Life Science,Heilongjiang University,Haerbin 150080,China)
关键词:
phytovirology;cytosinpeptidemycin;TMV; inactivation
摘  要:
Cytosinpeptidemycin was a newly reported anti-phytoviral agricultural antibiotic.It strongly inactivated virions of TMV in vitro and lowered the infectivity of TMV,which could not be recovered by diluting the mixture of TMV and cytosinpeptidemycin.The virions treated with cytosinpeptidemycin showed ruptures and abnormality under electron microscope,which indicated that cytosinpeptidemycin could destruct the structure of TMV.The results of Indi-ELISA also showed that cytosinpeptidemycin greatly reduced the concentration of TMV in infected tobacco leaves.

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