关键词：
唐古特白刺果实;DPPH自由基;ABTS+·;抗氧化性;分离提取
摘 要：
采用95%乙醇加热回流提取唐古特白刺(Nitraria tangutorum Bohr.)果实成分,依次用石油醚、氯仿、乙酸乙酯和正丁醇进行萃取。用DPPH自由基法和ABTS+·法进行抗氧化活性实验,并选取抗坏血酸(VC)和生育酚(VE)作为对照。结果显示乙酸乙酯提取物(EAF)具有较高的抗氧化性,进一步对其进行硅胶柱层析分离,得到6个组分(EAF-Ⅰ～EAF-Ⅵ),其中EAF-Ⅰ和EAF-Ⅳ的抗氧化性均高于VC和VE。白刺果实提取物具有良好的抗氧化性,有进一步开发为天然抗氧化剂的应用前景。
译 名：
Antioxidant Activity of Extracts from Nitraria tangutorum Bohr. Fruits
作 者：
REN Xu,CHEN Gui-lin(College of Life Science,Inner Mongolia University,Hohhot 010021,China)
关键词：
Nitraria tangutorum Bohr. fruit;DPPH free radical;ABTS+ free radical;antioxidant activity;separation and purification
摘 要：
Nitraria tangutorum Bohr. fruits were extracted using 95% ethanol,and then the extract was partitioned with petroleum ether,chloroform,ethyl acetate and n-butanol successively. The antioxidant activities of different fractions were evaluated by scavenging capability of ABTS+ and DPPH free radicals,and compared with the antioxidant activities of ascorbic acid(vitamin C) and tocopherol(vitamin E) . Results indicated that ethyl acetate fraction(EAF) exhibited the highest antioxidant activity. EAF was further fractionated into 6 fractions by silica gel column chromatography. The antioxidant activities of EAF-I and EAF-IV were higher than those of vitamin C and vitamin E. Therefore,N. tangutorum fruit extracts have excellent antioxidant activities,and have promising application prospects as a natural antioxidant.
