中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > N-乙酰半胱氨酸对玻璃化冻存小鼠GV期卵母细胞成熟和后续胚胎发育能力影响 东北农业大学学报 2017,48 (2) 76-82
Position: Home > Articles > Effect of N-acetyl-L-cysteine on mouse GV oocyte maturation and subsequent embryonic development following vitrfication Journal of Northeast Agricultural University 2017,48 (2) 76-82

N-乙酰半胱氨酸对玻璃化冻存小鼠GV期卵母细胞成熟和后续胚胎发育能力影响

作  者：
周佳勃;张宇霆;岳巍;邢月超;汪顺伟;孙梦;岳顺利
单  位：
黑龙江省动物细胞与遗传工程重点实验室东北农业大学生命科学学院
关键词：
小鼠;N-乙酰半胱氨酸;玻璃化冷冻保存;卵母细胞;ROS
摘  要：
冷冻保存后的GV期卵母细胞内大量积累ROS,影响胚胎发育。NAC作为一种抗氧化剂,可缓解氧应激造成的损伤。但NAC对冷冻GV期卵母细胞是否存在维持作用尚待研究。为探究NAC在冷冻、解冻后小鼠GV期卵母细胞发育中作用,检测冷冻保存后小鼠GV期卵母细胞在添加不同浓度NAC成熟培养液后成熟情况,观察其卵裂率与囊胚率、线粒体分布、ROS水平和受精后胚胎发育等。结果表明,添加1.5 mmol·L~(-1)NAC,冷冻保存后,小鼠GV期卵母细胞,线粒体排布均匀,卵母细胞比例显著提高,ROS水平降低。NAC可改善小鼠GV期卵母细胞经冷冻保存后复苏和体外成熟水平,提高其发育能力。
译  名：
Effect of N-acetyl-L-cysteine on mouse GV oocyte maturation and subsequent embryonic development following vitrfication
作  者：
ZHOU Jiabo;ZHANG Yuting;YUE Wei;XING Yuechao;WANG Shunwei;SUN Meng;YUE Shunli;Key Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province,School of Life Sciences,Northeast Agricultural University;
关键词：
mouse;;N-acetyl-L-cysteine;;vitrification;;oocyte;;ROS
摘  要：
Large amounts of ROS were accumulated in frozen-thawed GV-stage oocytes, which could affect embryonic development. NAC, as an antioxidant, could alleviate the damage caused by oxidative stress. However, currently, it remained to be seen whether NAC has a maintenance effect in frozen-thawed GV-stage oocytes. To investigate the role of NAC in frozen-thawed mouse GV-stage oocytes development, the maturation of frozen-thawed mouse GV-stage oocytes was examined at maturation medium added different concentrations of NAC and the cleavage rate, blastocyst rate,mitochondrial distribution, ROS level and embryo development after fertilization were observed. The results showed that the mitochondria were homogeneous, the proportion of oocytes was significantly increased, and the level of ROS was decreased into cryopreserved mouse GV-stage oocytes after adding 1.5 mmol · L~(-1) NAC. These results suggested that NAC can improve the recovery and in vitro maturation of frozen-thawed mouse GV-stage oocytes and improve their developmental potency.

相似文章

导出引用
计量
文章访问数: 1
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46