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Position: Home > Articles > 抗虫耐除草剂转基因玉米花粉生活力研究 Journal of Maize Sciences 2019 (6) 58-61

抗虫耐除草剂转基因玉米花粉生活力研究

作  者:
刘来盘;刘标
关键词:
转基因玉米;Cry1Ab;EPSPS;花粉萌发;基因漂移
摘  要:
使用离体培养法检测不同玉米材料花粉萌发率并于显微镜下观察花粉粒直径,研究转Cry1Ab、EPSPS基因玉米对花粉生活力的影响.结果表明,刚离体花粉萌发活力最强,培养180 min后花粉萌发率可达89.4%,高温(36℃)条件下放置2 h花粉基本丧失萌发能力.与非转基因对照相比,转Cry1Ab、EPSPS基因玉米对花粉离体后的生存能力无显著影响,对花粉粒直径大小无影响.
作  者:
LIU Lai-pan;LIU Biao;College of Life Science, Nanjing University;Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment;
关键词:
Transgenic maize;;Cry1Ab;;EPSPS;;Pollen germination;;Gene flow
摘  要:
In order to determine the effect of Cry1Ab and EPSPS gene maize on the pollen viability, the pollen germination rate of different maize materials was detected by the method of vitro culture and the diameter of pollen grains was observed under the microscope. The results showed that pollen germination rate of maize was the strongest when the pollen just in vitro which approximately 89.4% after 180 min. The pollen was almost completely inactivated when put it at high temperature(36℃) for 120 minutes. No significant effect on the viability of pollen in vitro and the diameter of pollen grains between the transgenic maize and the normal control.

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