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当前位置: 首页 > 文章 > 促红细胞生成素对大鼠视网膜缺血再灌注损伤中脑红蛋白表达的作用 石河子大学学报(自然科学版) 2012,30 (4) 87-91
Position: Home > Articles > Effect of Erythropoietin on the Expression of Neuroglobin in Rats after Retinal Ischemia-Reperfusion Injury Journal of Shihezi University(Natural Science) 2012,30 (4) 87-91

促红细胞生成素对大鼠视网膜缺血再灌注损伤中脑红蛋白表达的作用

作  者：
赵晨;张奕霞
单  位：
石河子大学医学院;石河子大学第一附属医院眼科
关键词：
促红细胞生成素;缺血再灌注损伤;脑红蛋白;细胞凋亡;视网膜
摘  要：
通过观察大鼠视网膜缺血再灌注后不同时间点视网膜细胞中脑红蛋白(Neuroglobin,NGB)的表达和视网膜细胞的凋亡情况及促红细胞生成素(Erythropoietin,EPO)干预的影响,研究大鼠视网膜缺血再灌注后视网膜损伤的机制及外源性EPO的保护作用。将84只SD大鼠随机分为正常对照组(D组,一共6只动物,仅行右眼前房穿刺)、模型对照组(A组,分为0、12、24、48、72h5组,每组6只动物)、生理盐水组(B组,分为12、24、48、72h4组,每组动物6只)、EPO组(C组,分组同B组),B组、C组动物于缺血再灌注模型建立后立即给予5μL生理盐水或EPO玻璃体腔注射,其它处理同A组。建立SD大鼠右眼缺血再灌注模型后,于相应时间点取视网膜组织待测。以脱氧核糖核昔酸末端转移酶介导的缺口末端标记法(TUNEL)检测各组视网膜细胞的凋亡指数(Apotosis index,AI);以免疫组化法检测各组视网膜细胞中NGB的表达。数据比较采用单因素方差分析。结果显示,D组未见视网膜凋亡细胞,NGB蛋白主要表达于视网膜神经节细胞层及内核层;与A、B组比较,缺血再灌注模型建立后不同时间段,C组凋亡指数均显著低于于A、B组,NGB蛋白表达均明显升高(均P<0.01);A组与B组NGB表达比较未见差异,与D组比较,除了A组0h相比D组表达降低(P>0.05)外,A、B组均高于D组(P<0.01),随时间增加,A、B组同组不同时间点凋亡指数升高,比较均有差异(P<0.05);C组凋亡指数及NGB蛋白表达24h组与48、72h组比较无差异,3组凋亡指数及NGB蛋白表达均高于12h组(P<0.01)。由此可知,外源性EPO可能通过升高视网膜细胞中NGB蛋白的表达从而抑制大鼠缺血再灌注损伤后视网膜细胞凋亡,对视网膜缺血再灌注损伤具有保护作用,该作用以缺血再灌注损伤给药后24h内作用最明显。
译  名：
Effect of Erythropoietin on the Expression of Neuroglobin in Rats after Retinal Ischemia-Reperfusion Injury
作  者：
ZHAO Chen1,ZHANG Yixia2(1 School of Medicine,Shihezi University,Shihezi 832000,China; 2 Department of Ophthalmology,School of Medicine,Shihezi University,Shihezi 832008,China)
关键词：
erythropoietin;ischemia-reperfusion injury;neuroglobin;apoptosis;retina
摘  要：
To observe the changes of levels of neurogloubin(NGB) protein and cell apoptosis after retinal ischemia-reperfusion injury in rats and to study the protective effects of erythropoietin(EPO) on cell apoptosis at different times.Method:A total of 84 female SD rats were randomly divided into 4 groups,namely normal control group(group D,n=6),model control group(group A,divided into five subgroups including 0h group,12h group,24h group,48h group and 72h group,n=6 each),saline group(group B,divided into four subgroups including 12h group,24h group,48h group and 72h group,n=6 each) and EPO group(group C,divided into four subgroups including 12h group,24h group,48h group and 72h group,n=6 each).The right eyes in group B received an intravitreal injection of 5 μl saline while the right eyes in group C received an intravitreal injection of 5 μl EPO after retinal ischemia-reperfusion injury immediately,other treatments as group A immediately.After establishing the retinal ischemia-reperfusion injury model,retinal samples were taken at the corresponding time for detecting retinal cells apoptosis by TUNEL method.The levels of NGB protein were measured by using immunohistochemistry.Experimental data were processed with one-factor variance analysis in SPSS package.Results:No apoptosis cells were observed in retinal tissues of group D and NGB protein predominantly expressed in ganglion cell layer and inner nuclear layer.Compared with group A and B,retinal apoptosis index decreased significantly in group C(P<0.01),and the levels of NGB protein increased significantly(P<0.01).The levels of NGB protein in group A and B were higher than that of group D(P<0.01),but between group A and group B there was no difference.The apoptosis index of group A and group B increased with time and the changes were significantly different(P<0.05).The apoptosis index and levels of NGB protein didn't show any obvious difference at 24h,48h and 72h in group C,but higher than that of 12h(P<0.01).Conclusion:Exogenous EPO,especially within 24 hours after the administration,could inhibit apoptosis of retinal cells and alleviate retinal damage after ischemia-reperfusion injury in rats.

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