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当前位置: 首页 > 文章 > 不同干燥方式对银耳多糖理化特性及抗氧化活性的影响 食品科学 2014,35 (13) 93-97
Position: Home > Articles > Effects of Different Drying Methods on Physio-chemical Properties and Antioxidant Activities of Polysaccharides Extracted from Tremella fuciformis FOOD SCIENCE 2014,35 (13) 93-97

不同干燥方式对银耳多糖理化特性及抗氧化活性的影响

作  者：
吴振;李红;罗杨;谭红军;陈岗;师萱;杨勇;詹永;石文娟
单  位：
重庆市食品药品检验所;重庆市银耳营养食品企业工程技术研究中心;重庆市中药研究院
关键词：
银耳多糖;干燥方式;理化特性;抗氧化活性
摘  要：
研究传统热风干燥、冷冻干燥和抽真空干燥等不同干燥方式对银耳多糖(Tremella fuciformispolysaccharides,TFPs)的理化特性和体外还原力、清除1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基、羟自由基(hydroxyl free radical,?OH)和超氧阴离子自由基(superoxide free radicals,O2-?)的影响。结果表明:3种干燥方式对TFPs均有显著影响,其中冷冻干燥处理多糖(freezing drying Tremella fuciformispolysaccharides,TFPs-F)的得率、总糖和黏度最高;3种干燥方式对粗多糖相对分子质量分布和和抗氧化活性有一定影响;TFPs-F的还原力、清除DPPH自由基、?OH和O2-?的半抑制浓度(half-inhibitory concentration,IC50)分别为2.61、1.64、1.78、1.75mg/mL;传统热风干燥和抽真空干燥处理多糖的还原力、清除DPPH自由基、?OH和O2-?的IC50高于低温冷冻干燥处理多糖相应的IC50。结果说明,冷冻干燥处理是获得高品质和高活性银耳多糖的较好方法。
译  名：
Effects of Different Drying Methods on Physio-chemical Properties and Antioxidant Activities of Polysaccharides Extracted from Tremella fuciformis
作  者：
WU Zhen;LI Hong;LUO Yang;TAN Hong-jun;CHEN Gang;SHI Xuan;YANG Yong;ZHAN Yong;SHI Wen-juan;HUANG Jing-yu;Chongqing Academy of Chinese Materia Medica;Chongqing Institute for Food and Drug Control;Chongqing Engineering Research Center for Tremella Nutrition Food Enterprises;
关键词：
Tremella fuciformis polysaccharides;;drying methods;;physio-chemical properties;;antioxidant activity
摘  要：
The infl uences of different drying methods, including traditional hot air drying, freeze drying and vacuum drying, on the physio-chemical properties and antioxidant activities of crude polysaccharides extracted from Tremella fuciformis(TFPs) were investigated. The results showed that freeze-dried TFPs(TFPs-F) had higher extraction effi ciency, total sugar content and viscosity. It was also found that molecular size distribution and antioxidant activities of TFPs were affected by three different drying methods. The IC50 for reducing power and scavenging activities against 1,1-diphenyl-2-picrylhydrazyl(DPPH free radical), hydroxyl and superoxide anion radicals of TFPs-F were 2.61, 1.64, 1.78, and 1.75 mg/mL, respectively. Moreover, it was clearly demonstrated that the IC50 of traditional hot air dried and vacuum dried TFPs was higher than that of low temperature freeze dried TFPs. The data obtained in vitro models suggest that freeze drying is an appropriate and effective way of obtaining the polysaccharides with antioxidant activity from Tremella fuciformis.

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