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Position: Home > Articles > Extraction Process for Components of Ginkgo biloba Ginkgolides and Bilobalide Northern Horticulture 2017 (23) 176-180

银杏叶片中萜内酯的提取工艺

作  者:
李泽宏;冯如;袁红慧;程华;程水源
单  位:
武汉轻工大学生物与制药工程学院
关键词:
银杏叶片;萜内酯;提取工艺
摘  要:
以银杏叶片为试材,采用乙醇-水浸提法,设计单因素试验,研究了乙醇浓度、提取时间、料液比、提取温度4个提取参数对萜内酯得率的影响,优化了提取工艺。结果表明:提取萜内酯的最佳工艺为料液比1∶150g·mL~(-1),提取温度60℃,60%乙醇,提取2.0h;最佳洗脱条件是石油醚∶乙酸乙酯=1∶2(v/v),一次性洗脱。经过HPLC-ELSD分析,该工艺提取率高,操作简便,具有可信度。
译  名:
Extraction Process for Components of Ginkgo biloba Ginkgolides and Bilobalide
作  者:
LI Zehong;FENG Ru;YUAN Honghui;CHENG Hua;CHENG Shuiyuan;School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University;
单  位:
School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University
关键词:
Ginkgo bilobaleaves;;ginkgolides and bilobalide;;extraction process
摘  要:
The effects of four extraction parameters on the ginkgolides and bilobalide yield were optimized by taking Ginkgo bilobaleaves as material,using ethanol-water extraction,single factor test.The ethanol concentration,extraction time,the ratio of solid-liquid and extraction temperature on the content of ginkgolides and bilobalide were studied.The extraction process was optimized.The results showed that the optimal extraction conditions were as follows,under the condition of the ratio of solidliquid 1∶150 g· mL~(-1),extraction temperature 60 ℃,extracting 2.0 hours with 60% ethanol.Petroleum ether∶ethyl acetate=2∶1(v/v).By the analysis of HPLC-ELSD,the extraction rate was high,simple,with credibility.

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