当前位置: 首页 > 文章 > 动物组织中β-兴奋剂类药物多残留酶联免疫检测方法的建立 河南农业科学 2013,42 (2) 132-135
Position: Home > Articles > Determination of β-agonists Multi-residue in Animal Tissue by Enzyme-linked Immunosorbent Assay Journal of Henan Agricultural Sciences 2013,42 (2) 132-135

动物组织中β-兴奋剂类药物多残留酶联免疫检测方法的建立

作  者:
万宇平;刘宁;陶光灿;吴鹏;李勇;崔廷婷
单  位:
临沂新程金锣肉制品集团有限公司;北京勤邦生物技术有限公司
关键词:
酶联免疫吸附法;β-兴奋剂类药物;多残留;动物组织
摘  要:
应用直接竞争酶联免疫吸附技术,建立了一种快速检测动物组织中β-兴奋剂类药物多残留的方法,并对其技术性能进行了评价.结果表明,该方法的IC50浮动范围为0.4~0.7μg/L,对组织样本的检测限为0.5μg/kg;样本添加回收率为72.9%~103.5%,变异系数为5.8%~14.1%;可用于检测克仑特罗、沙丁胺醇、塞布特罗、妥布特罗、克仑潘特、溴布特罗、溴氯布特罗、马喷特罗.
译  名:
Determination of β-agonists Multi-residue in Animal Tissue by Enzyme-linked Immunosorbent Assay
单  位:
Beijing Kwinbon Biotechnology Co.,Ltd.,Beijing 102206,China%Linyi Xincheng Jinluo Meat Products Co.,Ltd.,Linyi 276036,China
关键词:
ELISA%β-agonists%multi-residue%animal tissue
摘  要:
A rapid method for detection of β-agonists multi-residue in animal tissue was established based on direct competitive enzyme-linked immunosorbent assay(ELISA).The results showed that the IC50 was 0.4-0.7 μg/L,and the detection limit for animal tissue was 0.5 μg/kg.Sample recoveries were 72.9%-103.5% and the coefficients of variation were 5.8%-14.1%.This method was suitable for detecting clenbuterol,salbutamol,cimbuterol,tulobuterol,clenpenterol,brombuterol,bromchlorbuterol,mapenterol.This method is rapid,sensitive,and reliable,which provides a convenient and accurate method for detection of β-agonists residue.

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