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当前位置: 首页 > 文章 > 一组纤维素-林丹分解菌复合系的稳定性研究 农业环境科学学报 2008,27 (06) 2489-2493
Position: Home > Articles > Stability of A Composite Microbial System for Degrading Cellulose and Lindane Journal of Agro-Environment Science 2008,27 (06) 2489-2493

一组纤维素-林丹分解菌复合系的稳定性研究

作  者：
牛俊玲;崔宗均;李国学
单  位：
郑州航空工业管理学院资源与环境研究所;中国农业大学农学与生物技术学院;中国农业大学资源与环境学院
关键词：
纤维素;林丹;复合菌系;稳定性
摘  要：
本试验通过对一组高效降解纤维素林丹的复合微生物菌系的性质、功能和菌种组成进行研究,以确定该菌系的稳定性。结果表明,该复合系能在较大的pH范围内保持高的纤维素林丹分解活性,不同培养代在培养过程中的pH均呈现先降后升的趋势;不同代次的复合系在培养第3d时对滤纸的分解率均达到了90%以上,在第7d时对林丹的降解率均达到50%以上,且差异很小,说明该复合菌系产酶及分解纤维素和林丹的能力也已经非常稳定;平板培养基培养证明该复合菌系全部由细菌组成,用变性梯度凝胶电泳(DGGE)法比较来源于不同培养代的16SrDNA条带,分析菌种组成的变化,结果显示,不同培养代的菌种组成的主要条带没有变化,在DGGE图上出现的菌种能够稳定存在。
译  名：
Stability of A Composite Microbial System for Degrading Cellulose and Lindane
作  者：
NIU Jun-ling1, CUI Zong-jun2, LI Guo-xue3 (1.Institute of Resources and Environmental Science, Zhengzhou Institute of Aeronautical Industry Management, Zhengzhou 450015, China; 2.College of Agronomy and Biotechnology , China Agricultural University , Beijing 100094, China; 3.College of Resources and Environmental Science, China Agriculture University, Beijing 100094, China)
关键词：
cellulose; lindane; composite microbial system; stability
摘  要：
The stability of a high-efficient composite microbial system for degrading cellulose and lindane was studied by testing its characters, function and composition of bacteria strains. The composite microbial system could keep a higher degrading capability in a wider range of pH, which helped to adapt the complex compost environment. The pH value change of each generation in the process of subculturing tended to be the same as decreasing earlier and then rising. The degradation ratios of the filter paper were all above 90% after cultivated 3 days for the different generations of the composite system, the degradation ratios of lindane were all reached to more than 50% , and there were little differences among them. The result showed that the composite microbial system was very stable in degrading cellulose and lindane and producing cellulase. Plate culture results showed that the composite microbial system was consisted of bacteria. The composition change of bacteria strain was studied by comparing the 16S rDNA bands from different generations through the technique of denaturing gradient gel electrophoresis(DGGE). It showed that the main DNA bands of different generations were not be changed, the bacteria strains showed in the DGGE plate could exist stably.

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