作 者：
樊肇胜;程佳;王利军;李丹丹;秦莉;张艳玲;董阿力德尔图;朝格图
关键词：
短瓣金莲花;抗氧化活性部位;黄色素;总黄酮;总酚;羟自由基;DPPH自由基
摘 要：
目的:研究短瓣金莲花的抗氧化活性部位。方法:将短瓣金莲花药材依次用石油醚、乙酸乙酯、95%乙醇、60%乙醇、30%乙醇和去离子水提取,采用紫外-可见分光光度法对不同溶剂提取部位的黄色素、总黄酮和总酚含量进行分析,并测试各提取部位对羟自由基(·OH)和1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基的清除作用以对其抗氧化活性进行研究。结果:短瓣金莲花石油醚提取部位的黄色素含量最高,为(11.37±0.07)mg/g;而60%乙醇提取部位总黄酮和总酚含量最高,分别为(213.21±1.12)mg/g和(121.75±0.58)mg/g,95%乙醇提取部位次之,分别为(200.47±0.65)mg/g和(105.19±0.61)mg/g。石油醚提取部位清除·OH能力最强,半抑制浓度(50%inhibiting concentration,IC50)为92.77 mg/L;95%乙醇提取部位清除DPPH自由基能力最强,IC50为10.14 mg/L,60%乙醇提取部位次之,IC50为10.70 mg/L。结论:短瓣金莲花清除·OH能力与黄色素含量呈正相关性,而清除DPPH自由基能力与黄酮和多酚含量呈正相关性;短瓣金莲花的石油醚和95%乙醇提取部位是最佳的抗氧化活性部位,有待进一步深入研究。
译 名：
Antioxidant Activity of Different Solvents Extracts from Flowers of Trollius ledebouri Reichb
作 者：
FAN Zhao-sheng;CHENG Jia;WANG Li-jun;LI Dan-dan;QIN Li;ZHANG Yan-ling;DONG Alideertu;Tsogt;School of Chemistry and Chemical Engineering, Inner Mongolia University;
关键词：
flowers of Trollius ledebouri;;antioxidant extracts;;yellow pigment;;total flavonoids;;total polyphenols;;hydroxyl radical;;DPPH radical
摘 要：
Purpose: To explore the antioxidant activity of different solvent extracts from the flowers of Trollius ledebouriReichb. Methods: The dried flowers were extracted sequentially with petroleum ether, ethyl acetate, 95% ethanol, 60% ethanol, 30% ethanol and deionized water. Each extract obtained was analyzed by ultraviolet-visible spectrophotometry for the contents of yellow pigment, total flavonoids and total polyphenols, and their radical scavenging activity against hydroxyl and DPPH free radicals were tested. Results: Petroleum ether extract had the highest content of yellow pigment(11.37 ±0.07) mg/g; 60% ethanol extract from the flowers of Trollius ledebouri had the highest content of total flavonoids(213.21 ±1.12) mg/g and total polyphenols(121.75 ± 0.58) mg/g, 95% ethanol extract had slightly lower contents of total flavonoids(200.47 ± 6.49) mg/g and total polyphenols(105.19 ± 0.61) mg/g. Petroleum ether extract had the strongest hydroxyl radical scavenging ability with IC50 of 92.77 mg/L, while 95% ethanol extract had the strongest DPPH radical scavenging ability with IC50 of 10.14 mg/L and 60% ethanol extract had slightly lower DPPH radical scavenging ability with IC50 of 10.70 mg/L. Conclusion: The hydroxyl radical scavenging capacity presented a positive correlation with the concentration of yellow pigment, and the DPPH radical scavenging capacity presented a positive correlation with the concentration of total flavonoids and total polyphenols; petroleum ether and 95% ethanol extracts from the flowers of Trollius ledebouri have potent antioxidant activity.
