译 名：
Inhibitory Effects of Three Compounds Extracted from Trollius ledebouri on Phenoloxidase of Spodotera exigua
作 者：
YANG Fan1,2,LIU Zhao-yang2,LIU Wei1,DU Lei1,XUE Chao-bin1,LUO Wan-chun1(1.College of Plant Protection,Shandong Key Laboratory of Pesticide Toxicology and Application Technique,Shandong Agricultural University,Tai′an 271018,Shangdong Province,China;2.Key Laboratory of Pharmaceutical Chemistry and Molecular Diagnosis,Ministry of Education,Baoding 073000,Hebei Province,China)
关键词：
Trollius ledebouri;extracts;phenoloxidase;inhibitory effects
摘 要：
In order to screen the effective inhibitors of phenoloxidase from Spodotera exigua,three compounds were isolated from the ethanol extracts of Trollius ledebouri with traditional isolation and preparative HPLC methods.The structures were determined to be methyl 3,4-dihydroxybenzoate(compoundⅠ),2″-O-(2″'methylbutyryl)vitexin(compound Ⅱ) and Ursolic acid(compound Ⅲ) based on spectral methods,respectively.In addition,the effects of the compounds on phenoloxidase(PO) activity of Spodotera exigua were also investigated.The results indicated that the three compounds showed different inhibitory effects on the PO and the inhibitor concentrations leading to 50%(IC50) activity lost were estimated to be 2.04 mmol/L for compound Ⅰ,0.83 mmol/L for compound Ⅱ and 0.32 mmol/L for compound Ⅲ,respectively.
