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Position: Home > Articles > Isolation,Identification and Characterization of a Bacterium with Highly Efficient Removal of Nitrite Nitrogen Fisheries Science 2018 (5) 689-693

一株去除亚硝态氮细菌分离鉴定及特性研究

作  者:
姚艳玲;袁春营;崔青曼;吕军
单  位:
松辽水环境科学研究所;天津科技大学海洋与环境学院
关键词:
异养硝化细菌;脱氮海洋单胞菌;分离鉴定;脱氮特性
摘  要:
自对虾养殖后期池水中分离纯化出一株高效去除亚硝态氮的细菌,进行了菌体的亚显微形态观察和16SrDNA同源序列分析,并探讨了起始pH、温度和碳氮比对该菌株去除亚硝态氮效果的影响。结果表明,分离菌株在初始亚硝态氮质量浓度为55.51mg/L的异养硝化培养基中培养12h后,亚硝态氮去除率达到98.69%;根据形态学特征、生理生化特性及16SrDNA序列分析,确定该菌株为脱氮海洋单胞菌,菌株去除亚硝态氮的最适宜条件为:初始pH 9,温度35℃,碳氮比16。
译  名:
Isolation,Identification and Characterization of a Bacterium with Highly Efficient Removal of Nitrite Nitrogen
作  者:
YAO Yanling;YUAN Chunying;CUI Qingman;LYU Jun;Songliao Institute of Water Environmental Science;College of Marine Science & Environment,Tianjin University of Science and Technology;
关键词:
heterotrophic bacterium;;Oceanimons denitrificans;;isolation and identification;;nitrogen removal characteristics
摘  要:
A bacterium eliminating nitrite nitrogen efficiently was isolated from water in a shrimp pond in post culture period,and identified by a transmission electron microscope,and by homology analysis of16 SrDNA sequence,and the effect of initial pH,temperature and carbon nitrogen ratio on the removal of nitrite nitrogen by the bacterial strain was investigated.The results showed that the strain had the removal rate of 99.79%for nitrite nitrogen in the heterotrophic nitrification medium with the initial nitrite nitrogen concentration of 55.51 mg/L 12 hafter culture.The strain was identified as Oceanimons denitrificans according to the morphological characteristics,physiological and biochemical characteristics and 16 SrDNA sequence analysis,and showed the maximal removal rate of nitrite nitrogen under the conditions of initial pH 9,35 ℃,and C/N of 16.

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