单 位：
仲恺农业工程学院化学化工学院;沃特世科技(北京)有限公司
关键词：
精确质量数;残留分析;QuEChERS方法;超高效液相色谱(UPLC);有机磷农药
摘 要：
建立利用超高效液相色谱-高分辨飞行时间质谱同时检测蔬菜中敌敌畏、毒死蜱、乐果、氧乐果4种有机磷农药残留的方法。试样经QuEChERS方法进行前处理,采用超高效液相色谱-高分辨飞行时间质谱法测定,基质匹配标准溶液外标法定量。4种有机磷农药在质量浓度10~250 ng/mL范围内线性关系良好(R2>0.99),检测限和定量下限分别为0.25~0.40μg/kg和0.73~1.00μg/kg,平均加标回收率为80%~98%,相对标准偏差为2.4%~9.7%。该方法简便、快捷、可靠、稳定,可用于蔬菜中敌敌畏、毒死蜱、乐果、氧乐果4种有机磷农药的快速检测。
译 名：
Determination of 4 pesticide residues in vegetable using QuEChERS extraction and UPLC-TOFMS
作 者：
LU Hong1,WU Guo-jie1,LIANG Shi-qiang1,BAI Xu1,MU Xiao-mei1(1.College of Chemistry and Chemical Engineering,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China; 2.Waters Technologies(Beijing) Limited,Beijing 100026,China)
关键词：
accurate mass;residual analysis;QuEChERS method;ultra performance liquid chromatography(UPLC);organophosphorus pesticide
摘 要：
An ultra performance liquid chromatography(UPLC)-high resolution time of flight mass spectrometer(TOFMS) method was established for the simultaneous determination of dichlorvos,chlorpyrifos,omethoate,and dimethoate residues in vegetable.The QuEChERS method was used for sample pretreatment.The matrix-matched external standard calibration curves were used for the quantitative analysis.The calibration curves of 4 organic phosphorous pesticides were linear in concentration range from 10 ng/mL to 250 ng/mL with correlation coefficients above 0.99.The limit of detection was 0.25~0.40 μg/kg and the limit of quantification was 0.73~1.00 μg/kg.The spiked recoveries in vegetable ranged from 80% to 98%.The relative standard deviations of the method were between 2.4% and 9.7%.This method was simple,efficient and accurate.Therefore,it was practicable in the trace determination of dichlorvos,chlorpyrifos,omethoate,and dimethoate in vegetable.
