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Position: Home > Articles > Study on Virus-free Technique of Chuzhou Chrysanthemum by Tissue Culture Journal of Anhui Agricultural Sciences 2007,35 (14) 56+85

滁菊组织培养脱病毒技术研究

作  者:
邓衍明;褚芳玲;华如枝
单  位:
安徽省滁州市农业科学研究所
关键词:
滁菊;组织培养;热处理;脱毒
摘  要:
采用改良热处理结合茎尖分生组织培养的方法,建立滁菊组培脱毒快繁技术体系。研究结果表明,滁菊茎段诱导的最佳培养基为MS+2.0 mg/L6-BA+0.1 mg/LNAA;茎尖增殖的最佳培养基为MS+1.0 mg/L6-BA+0.1 mg/LNAA;壮苗生根的最佳培养基为1/2MS+0.1 mg/LNAA。接种脱毒结果为,脱毒率>83%,茎尖成活率>42%。
译  名:
Study on Virus-free Technique of Chuzhou Chrysanthemum by Tissue Culture
作  者:
DENG Yan-ming et al(Agricultural Science Institute of Chuzhou,Chuzhou,Anhui 239000)
关键词:
Chuzhou Chrysanthemum;Tissue culture;Heating treatment;Virus-free
摘  要:
Rapid propagation technical system of the virus-free plantlets of Chuzhou Chrysanthemum was established by shoot tip meristematic tissue culture combined with improved heating treatment.The optimum stem induction medium was MS + 2.0 mg/L 6-BA + 0.1 mg/L NAA.The optimum shoot tip propagation medium was MS + 1.0 mg/L 6-BA + 0.1 mg/L NAA.The optimum rooting medium was 1/2 MS +0.1 mg/L NAA.The virus-free ratio of shoot tips was above 83 % and the survival ratio was above 42 %.

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