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当前位置: 首页 > 文章 > 产复合酶枯草芽孢杆菌QLB6的激光诱变育种 食品科学 2011,32 (9) 222-224
Position: Home > Articles > Breeding of a Mutant Strain of Bacillus subtilis QLB6 with Higher Proteolytic Activity by He-Ne Laser Irradiation FOOD SCIENCE 2011,32 (9) 222-224

产复合酶枯草芽孢杆菌QLB6的激光诱变育种

作  者：
宋鹏;黄亮;陈亮
单  位：
河南工业大学生物工程学院;河南科技大学农学院
关键词：
枯草芽孢杆菌;复合酶;He-Ne激光;遗传稳定性
摘  要：
以产复合酶枯草芽孢杆菌QLB6为出发菌株,利用氦-氖(He-Ne)激光进行诱变育种。通过计算存活率和正突变率,确定激光诱变育种的照射剂量。通过透明圈筛选和酶活力测定,筛选获得1株编号为QLB6F的菌株,其纤维素酶、蛋白酶、淀粉酶酶活力相对于出发菌株分别提高219%、38%和71%。进行30代遗传稳定性实验,证明它具有良好的遗传稳定性,无回复突变。
译  名：
Breeding of a Mutant Strain of Bacillus subtilis QLB6 with Higher Proteolytic Activity by He-Ne Laser Irradiation
作  者：
SONG Peng1,HUANG Liang2,CHEN Liang2(1.College of Agriculture,Henan University of Science and Technology,Luoyang 471003,China;2.School of Bioengineering,Henan University of Technology,Zhengzhou 450001,China)
关键词：
Bacillus subtilis;complex enzymes;He-Ne laser;genetic stability
摘  要：
In this study,Bacillus subtilis QLB6 that had been isolated from aged tobacco leaves and been found to have higher proteolytic activity was mutagenized by means of He-Ne laser irradiation.The optimum irradiation dose was determined based on survival rate and positive mutation rate.Based on transparent circle and proteolytic acivity,a target strain was obtained and named QLB6F.The strain exhibited an increase of 219%,38% and 71% respectively in cellulose,protease and amylase activities when compared to QLB6.After the 30th passage,the genetic stability of the mutant strain remained excellent and no reverse mutation was observed.

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