单 位：
江苏省农科院农产品加工研究所;中国农业科学院蜜蜂研究所;北京联合大学应用文理学院生物系
摘 要：
茶花花粉蛋白经过风味酶(外切酶)和中性蛋白酶(内切酶)组成的复合酶水解得到活性肽。通过单因素试验及正交试验得到各因素的优化组合,结果表明,复合酶的最佳水解工艺为:水解pH7,水解温度40℃,加酶量8%,两种酶比2:1,水解时间12h。此工艺水解所测得的茶花花粉蛋白水解度为40%,溶解指数为0.8,水解效果明显改善。
译 名：
Research on Optimal Process of Enzymatic Hydrolysis of Protein from Camellia Pollen
作 者：
DONG Jie1,LI Hui-yan1,ZHANG Hong-cheng1,,LI Chun-yang2,WEN Jing3 (1.Institute of Apicultural Research, Chinese Academy of Agricultural Science, Beijing 100093, China; 2.Institute of Agro-food Science and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 3.Department of Biology, College of Applied Arts and Science, Beijing Union University, Beijing 100083, China)
关键词：
camellia pollen;complex-enzyme;enzymatic hydrolysis
摘 要：
The complex of flavourzyme and neutral protease was used to hydrolyze camellia pollen to obtain bioactive peptides. The optimal process of enzymatic hydrolysis was determinated by orthogonal test. The test results indicated that the optimal conditions of enzymatic hydrolysis are pH value 7, temperature 40 ℃, amount added 8%, ratio of flavourzymeto neutral protease 2:1, time of hydrolysis 12 h. Under optimal conditions, the degree of hydrolysis is 40% and the nitrogen solution index is 0.8. The effect of hydrolysis was improved obviously.
